MHC Class II RT-1B 抗体
适用: 大鼠
FACS, IHC (fro), IHC (p), WB
宿主: 小鼠
Monoclonal
OX-6
unconjugated
1 image
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- 抗原 See all MHC Class II RT-1B products
- MHC Class II RT-1B
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MHC Class II RT-1B antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
- 纯化方法
- Affinity Chromatography on Protein G
- 免疫原
- Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- 克隆位点
- OX-6
- 亚型
- IgG1
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anti-MHC Class II RT-1B antibody
适用: 大鼠, 小鼠 FACS, IHC (fro), IHC (p), WB, IF 宿主: 小鼠 Monoclonal OX-6 unconjugated
anti-MHC Class II RT-1B antibody适用: 大鼠, 小鼠 FACS, IHC (fro), IHC (p), IHC, WB 宿主: 小鼠 Monoclonal OX-6 unconjugated
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- 应用备注
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Flow cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemsitry on paraffin sections: 1/50 - 1/100, This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6, This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde). Western Blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- MHC Class II RT-1B
- 别名
- MHC Class II RT1B (MHC Class II RT-1B 产品)
- 背景
- Synonyms: HLA Class II, MHC Class 2 RT1B
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