MHC Class II RT-1B 抗体
适用: 大鼠
FACS, IHC (fro), IHC (p)
宿主: 小鼠
Monoclonal
OX-6
unconjugated
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- 抗原 See all MHC Class II RT-1B products
- MHC Class II RT-1B
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MHC Class II RT-1B antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Affinity Chromatography on Protein G
- 免疫原
- Rat thymocyte membrane glycoprotein. Spleen cells from immunised BALB/c mice were fused with cells of the NS1 mouse myeloma cell line.
- 克隆位点
- OX-6
- 亚型
- IgG1
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anti-MHC Class II RT-1B antibody
适用: 大鼠, 小鼠 FACS, IHC (fro), IHC (p), WB, IF 宿主: 小鼠 Monoclonal OX-6 unconjugated
anti-MHC Class II RT-1B antibody适用: 大鼠, 小鼠 FACS, IHC (fro), IHC (p), WB, IHC 宿主: 小鼠 Monoclonal OX-6 unconjugated
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- 应用备注
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Flow Cytometry: 1/50 - 1/100, Use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. Immunohistochemistry on frozen sections. Immunohistochemistry on paraffin sections: 1/50 - 1/100, This product does not require antigen retrieval using heat treatment or protein digestion prior to staining of formalin-fixed paraffin-embedded sections, but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6, This clone has also been described reacting with paraffin-embedded material following PLP fixation (periodate-lysine-paraformaldehyde).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- MHC Class II RT-1B
- 别名
- MHC Class II RT1B (MHC Class II RT-1B 产品)
- 背景
- The major histocompatibility complex (MHC) is a cluster of genes that are important in the immune response to infections. In rats, this complex is referred to as the RT1 region. In mice, this complex is referred to as the H2 region.Synonyms: HLA Class II, MHC Class 2 RT1B
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