MHC Class II RT-1B 抗体
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- 抗原 See all MHC Class II RT-1B products
- MHC Class II RT-1B
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适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MHC Class II RT-1B antibody is un-conjugated
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Protein G affinity chromatography
- 免疫原
- Rat thymocyte membrane glycoproteins
- 克隆位点
- OX-6
- 亚型
- IgG1
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- 应用备注
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Flow cytometry (1:50 - 1:100): use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. This product is routinely tested in flow cytometry on rat splenocytes. Immunohistochemistry on frozen tissues and paraffin embedded sections (1:50 - 1:100): this clone has been described reacting with paraffin-embedded material following PLP fixation (periodatelysine-paraformaldehyde) (4). This product does not require antigen retrieval using heat treatment or protein digestion prior to staining but results may be enhanced with heat treatment using 0.01M citrate buffer pH 6. Western blot.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS, pH 7.2, containing 0.09 % sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
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- 抗原
- MHC Class II RT-1B
- 别名
- MHC Class II RT1B (MHC Class II RT-1B 产品)
- 背景
- Synonyms: HLA Class II, MHC Class 2 RT1B
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