Flow cytometry: 1/100, use 10 μL of the suggested working dilution to label 10^6 cells in 100 μL. (This product is routinely tested in flow cytometry on Lewis rat splenocytes). Immunohistochemistry on frozen and paraffin sections: This product does not require protein digestion pre-treatment of paraffin embedded sections e.g. trypsin or pronase prior to staining, This product does not require antigen retrieval using heat treatment methods prior to staining of paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4, containing 0.09 % Sodium Azide and 1 % Bovine Serum Albumin
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.