The monoclonal antibody is directed against the CDw49f-antigen. This monoclonal NKI-GoH3 antibody reacts with platelets, megakaryocytes, T lymphocytes and common Acute Lymphoblastic Leukaemia cells (alpha-6 beta-1). In immunohistology the monoclonal antibody reacts with epithelial cells of a variety of tissues, peripheral nerves, microvascular endothelial cells, placenta cyto- and syncytiotrophoblasts. Platelet VLA-6 functions as a laminin receptor and platelet adhesion to laminin is inhibited by GoH3. Studies suggest that Clone NKI-GoH3 specifically blocks the binding of cells to the E8 fragment of laminin (See Sonnenberg, A. et al. for details).
纯化方法
Affinity Chromatography on Protein G
免疫原
BALB/c mouse mammary tumour cells. Spleen cells from immunised Sprague-Dawley rats were fused with cells of the SP2/0 mouse myeloma cell line.
Flow cytometry. Immunohistochemistry on frozen sections. Recommended positive control: Human Tonsil. Immunoprecipitation. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
PBS, pH 7.4, 0.09 % Sodium Azide
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
CDw49f-antigen is a 120kD, (GP Ic or VLA-6 alpha-chain), which can form distinct complexes with either the CD29-antigen (GP 11a or VLA beta-chain), resulting in the VLA-6 (alpha-6 beta-1) complex, which is expressed on human platelets, or with the beta-4 integrin resulting in the alpha-6 beta-4 complex which is expressed on various human epithelial cells.Synonyms: CD49 antigen-like family member F, Integrin alpha-6, VLA-6, VLA6