我们推荐在您的浏览器上开启Javascript，以获得最佳效果。

CD4 抗体 (Non-Polymorphic Site) (FITC)

Name: CD4 抗体 (Non-Polymorphic Site) (FITC)
SKU: ABIN118164
Availability: OutOfStock

CD4 适用: 小鼠 FACS 宿主: 大鼠 Monoclonal YTS177-9 FITC

Staining of mouse peripheral blood lymphocytes with RAT ANTI MOUSE CD4:FITC (CD4 抗体 (Non-Polymorphic Site) (FITC))

FACS

1 image

产品编号 ABIN118164

发货至: 中国

Datasheet as PDF

抗原 See all CD4 抗体

CD4
抗原表位
27

16

16

16

16

10

8

7

5

4

4

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
Non-Polymorphic Site
适用
334

198

117

48

24

20

19

18

18

12

10

10

10

10

10

9

9

9

8

5

4

3

1

1

1

1

1
小鼠
宿主
341

153

108

3

3

1
大鼠
Compatible Secondaries
克隆类型
462

138

6
单克隆
标记
215

71

63

45

33

13

12

11

9

9

9

8

5

4

4

4

4

4

4

4

4

4

4

4

4

4

4

3

3

2

2

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1
This CD4 antibody is conjugated to FITC
应用范围
487

195

128

108

94

76

69

42

39

34

33

28

10

9

9

7

6

6

5

3

3

3

2

2

2

2

2

2

2

1

1

1

1

1

1
Flow Cytometry (FACS)

特异性

This antibody reacts with the CD4 antigen, non polymorphic epitope. The antibody is reported to block MHC class II dependant T-cell responses in vitro and in vivo and induces tolerance.

纯化方法

Affinity Chromatography on Protein G.

免疫原

Mouse spleen cells. Spleen cells from immunised DA rats were fused with cells of the Y3/Ag1.2.3 rat myeloma cell line.

克隆位点

YTS177-9

亚型

IgG2a

Top Product

Discover our top product CD4 Primary Antibody
anti-CD4 (CD4) (AA 385-457) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, Pig FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 适用: 人, 小鼠, 大鼠, 小鸡, 猴, 犬, Pig, Cat, Cow, 兔, 绵羊, 豚鼠, 山羊, 马, Avian, 驴, Hamster WB 宿主: 山羊 Polyclonal unconjugated

anti-CD4 (CD4) (AA 231-330) antibody
CD4 适用: 人, 小鼠, 大鼠 FACS, WB, ELISA, IHC (fro), IHC (p), IF (cc), IF (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) (N-Term) antibody
CD4 适用: 人 FACS, WB, CyTOF 宿主: 小鼠 Monoclonal MEM-241 unconjugated

anti-CD4 (CD4) (AA 300-400) antibody
CD4 适用: 人 WB, IF 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) (AA 301-400) antibody
CD4 适用: 人, 小鼠, 大鼠 FACS, WB, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-CD4 (CD4) antibody
CD4 适用: 小鼠 FACS, IP, IHC (fro), ICC, Func 宿主: 大鼠 Monoclonal GK1-5 unconjugated

anti-CD4 (CD4) (N-Term) antibody (FITC)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 FITC

anti-CD4 (CD4) (N-Term) antibody (PE)
CD4 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-241 PE

anti-CD4 (CD4) antibody (PE)
CD4 适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-35 PE

应用备注

Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells or 100 μL whole blood. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

0.1 mg/mL

缓冲液

PBS, pH 7.2 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.

储存条件

4 °C/-20 °C

储存方法

Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
抗原

CD4

别名

CD4 (CD4 产品)

背景

CD4 is a single chain transmembrane glycoprotein and belongs to immunoglobulin supergene family. In extracellular region there are 4 immunoglobulin-like domains (1 Ig-like V-type and 3 Ig-like C2-type). Transmembrane region forms 25 aa, cytoplasmic tail consists of 38 aa. Domains 1,2 and 4 are stabilized by disulfide bonds. The intracellular domain of CD4 is associated with p56Lck, a Src-like protein tyrosine kinase. It was described that CD4 segregates into specific detergent-resistant T-cell membrane microdomains. Extracellular ligands: MHC class II molecules (binds to CDR2-like region in CD4 domain 1), HIV envelope protein gp120 (binds to CDR2-like region in CD4 domain 1), IL-16 (binds to CD4 domain 3), Human seminal plasma glycoprotein gp17 (binds to CD4 domain 1), L-selectin Intracellular ligands: p56Lck CD4 is a co-receptor involved in immune response (co-receptor activity in binding to MHC class II molecules) and HIV infection (human immunodeficiency virus, CD4 is primary receptor for HIV-1 surface glycoprotein gp120). CD4 regulates T-cell activation, T/B-cell adhesion, T-cell diferentiation, T-cell selection and signal transduction. Defects in antigen presentation (MHC class II) cause dysfunction of CD4+ T-cells and their almost complete absence in patients blood, tissue and organs (SCID immunodeficiency).Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

基因ID

12504

NCBI登录号

NP_038516

UniProt

P06332

途径

TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events