CD19 抗体 (PE)
-
- 抗原 See all CD19 抗体
- CD19 (CD19 Molecule (CD19))
-
适用
- 小鼠
-
宿主
- 大鼠
-
克隆类型
- 单克隆
-
标记
- This CD19 antibody is conjugated to PE
-
应用范围
- Flow Cytometry (FACS)
- 特异性
- This antibody recognises the CD19 cell surface antigen, a 95 kDa glycoprotein expressed by B lymphocytes. Clone 6D5 has been shown to recognise the same epitope as clone ID3 in cross-competition assays.
- 纯化方法
- Affinity chromatography on Protein G
- 免疫原
- Human K562 cell line transfected with murine CD19. Spleen cells from immunised rats were fused with cells of the P3X63.Ag8.653 myeloma cell line.
- 克隆位点
- 6D5
- 亚型
- IgG2a
- Top Product
- Discover our top product CD19 Primary Antibody
-
-
- 应用备注
-
Flow Cytometry: Use 10 μL of Neat-1/10 diluted antibody to label 10^6 cells in 100 μL. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
-
- 溶解方式
- Restore with 1 mL distilled water
- 缓冲液
- PBS, pH 7.4 containing 0.09 % Sodium Azide as preservative and 1 % BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- This product is photosensitive and should be protected from light
- 储存条件
- 4 °C
- 储存方法
- Prior to and following reconstitution store the antibody at 2-8 °C. DO NOT FREEZE!
-
- 抗原
- CD19 (CD19 Molecule (CD19))
- 别名
- CD19 (CD19 产品)
- 背景
- CD19 is a member of the immunoglobulin superfamily and has two Ig like domains. The CD19 Molecule is expressed on 100 % of the peripheral B cells as defined by expression of kappa or lamda light chains. It is expressed on approximately 10 % of normal human peripheral blood cells and approximately 60 % of splenic lymphocytes. It is not expressed on granulocytes, monocytes or T cells as defined by CD3 expression. CD19 defines a pan B antigen which is expressed from the earliest stages of B progenitor development, but is lost on terminal differentiation to plasma cells. It may also be present on some early myeloid progenitors, particularly those of the monoblastic type. The CD19 antigen is expressed on approximately 12 % of peripheral blood lymphocytes. It appears to be expressed on myeloid leukemia cells, particularly those of monocytic lineage. Leukemia phenotype studies have demonstrated that the earliest and broadest B cell restricted antigen is the CD19 antigen.Synonyms: B-cell marker, B-lymphocyte surface antigen B4, Differentiation antigen CD19, Leu-12
- 基因ID
- 12478
- NCBI登录号
- NP_033974
- UniProt
- P25918
- 途径
- Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
-