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- 抗原 See all ATG7 抗体
- ATG7 (ATG7 Autophagy Related 7 (ATG7))
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抗原表位
- AA 558-569
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This ATG7 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human Apg7 protein. However, BLAST analysis indicates 100 % homology (12/12) for this protein from human, chimpanzee, mouse, rat, dog, and chicken sources. The core amino acids of the immunogen, G-D-S-T-R-D-R-T are 100 % identical (8/8) in D. melanogaster. Reactivity with Apg7 proteins from other sources is not known.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding aa 558-569 of Human Apg7 protein.
- Top Product
- Discover our top product ATG7 Primary Antibody
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- 应用备注
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This affinity purified antibody has been tested for use in ELISA (1: 15,000-1: 75,000) and byWestern blot (1: 500- 1: 2,000). Specific conditions for reactivity should be optimized by theend user. Expect a band approximately 78 kDa in size corresponding to human Apg7 bywestern blotting in the appropriate cell lysate or extract. Human Apg7 has been reported toform a homodimer at approximately 160 kDa.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 浓度
- 1.46 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Dilute only prior to immediate use
- 储存条件
- -20 °C
- 储存方法
- Store vial at -20° C prior to opening. For extended storage aliquot contents and freeze at -20 °C or below. Avoid cycles of freezing and thawing.
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[A difficult case of esophageal and gastric double cancer with pleural and pericardial effusion following chemo-radiotherapy (CRT)]" in: Gan to kagaku ryoho. Cancer & chemotherapy, Vol. 34, Issue 12, pp. 1979-81, (2008) (PubMed).
: "[Relation between acute respiratory infection and enterovirus in children in Beijing area.]" in: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology, Vol. 21, Issue 4, pp. 316-8, (2008) (PubMed).
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[A difficult case of esophageal and gastric double cancer with pleural and pericardial effusion following chemo-radiotherapy (CRT)]" in: Gan to kagaku ryoho. Cancer & chemotherapy, Vol. 34, Issue 12, pp. 1979-81, (2008) (PubMed).
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- 抗原
- ATG7 (ATG7 Autophagy Related 7 (ATG7))
- 别名
- APG7L / ATG7 (ATG7 产品)
- 背景
- Apg7 is the human gene product similar in homology to Pichia pastoris GSA7 and Saccharomyces cerevisiae Apg7. In the yeast, the protein appears to be required for fusion of peroxisomal and vaculuolar membranes. The protein shows homlogy to the ATP-binding and catalytic sites of the E1 ubiquitin activating enzymes.Synonyms: APG7-like, Autophagy-related protein 7, Ubiquitin-activating enzyme E1-like protein
- 基因ID
- 10533
- NCBI登录号
- NP_001129503
- UniProt
- O95352
- 途径
- Response to Water Deprivation, Autophagy
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