This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p33 ING1 protein and is 100 % homologous to all four isoforms (A, B, C, and D) on record for this protein. Cross reactivity with p33 ING1 protein from mouse and rat sources is expected based on high homology (one amino acid change in sequence) by BLAST. Reactivity with p33 ING1 proteins from other sources is not known.
纯化方法
Immunoaffinity chromatography.
免疫原
This affinity purified antibody was prepared from whole Goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 285-296 of Human p33 ING1 protein (Inhibitor of growth family, member 1). Remarks: This sequence shows 100% homology to all splice variants for ING1.
This affinity-purified antibody has been tested for use in ELISA (1: 4,000-1: 14,000) andWestern blotting (1: 500-1: 3,000) using over expressed cell lysates. Expect a bandapproximately 47 kDa, 33 kDa or 24 kDa in size corresponding to variants of ING1 bywestern blotting in the appropriate cell lysate or extract. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL (UVabs. 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, with 0.01 % Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件
-20 °C
储存方法
Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
抗原
ING1
(Inhibitor of Growth Family, Member 1 (ING1))
p24ING1c antibody, p33 antibody, p33ING1 antibody, p33ING1b antibody, p47 antibody, p47ING1a antibody, p33ING1c antibody, zgc:136918 antibody, ING1 antibody, 2610028J21Rik antibody, AA407184 antibody, AI875420 antibody, mING1h antibody, p33Ing1 antibody, p37Ing1b antibody, xing1 antibody, inhibitor of growth family member 1 antibody, inhibitor of growth family, member 1 antibody, inhibitor of growth family member 1 S homeolog antibody, ING1 antibody, Ing1 antibody, ing1 antibody, ing1.S antibody
背景
P33 ING1 is a tumor suppressor protein that can induce cell growth arrest and apoptosis. The encoded protein is a nuclear protein that physically interacts with the tumor suppressor protein TP53 and is a component of the p53-signaling pathway. Reduced expression and rearrangement of this gene have been detected in various cancers. Multiple alternatively spliced transcript variants encoding distinct isoforms have been reported. The accession number listed below is for variant (4) that encodes the longest isoform (D). Other synonyms for p33 ING1 include p33, p47, p33ING1, p24ING1c, p33ING1b, p47ING1a, growth inhibitor ING1, inhibitor of growth 1, tumor suppressor ING1 and growth inhibitory protein ING1.Synonyms: ING-1, Inhibitor of growth protein 1