This is an affinity purified antibody produced by immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human p28 ING5 protein. The sequence is present in both isoforms 1 and 2 (see below). However, 100 % homology is on record for this protein from human and chimpanzee. Cross reactivity with p28 ING5 protein from frog, mouse, rat and chicken may not occur as sequence homology varies by one or more amino acids residues in this sequence as indicated by BLAST analysis. Reactivity with p28 ING5 proteins from other sources is not known.
纯化方法
Immunoaffinity chromatography.
免疫原
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 127-140 of Human p28 ING5protein (Inhibitor of growth family, member 5). This sequence shows homology to isoforms 1 and 2 for ING5.
This affinity purified antibody has been tested for use in ELISA (1: 4,000 - 1: 16,000) and byWestern blot (1: 500-1: 1,000, Expect a band approximately 28 kDa or 26 kDa in sizecorresponding to variants of ING5 in the appropriate cell lysate or extract). Also suitable for Immunofluorescence microscopy.
限制
仅限研究用
浓度
1.2 mg/mL (UVabs. 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件
-20 °C
储存方法
Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
抗原
ING5
(Inhibitor of Growth Family, Member 5 (ING5))
ING5 is similar to ING1, a tumor suppressor protein that can interact with TP53, inhibit cell growth, and induce apoptosis. ING5 is a component of the HBO1 complex which has a histone H4-specific acetyltransferase activity, a reduced activity toward histone H3 and is responsible for the bulk of histone H4 acetylation in vivo. ING5 acts as a tumor suppressor and is a potent growth regulator in normal and in established cells. ING5 may have involvement in chromatin remodeling.Synonyms: Inhibitor of growth protein 5, p28ING5