Immunoaffinity Chromatography using the immunizing peptide after immobilization to a solid phase.
免疫原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to aa 3-14 of Human MAD2L2.
This affinity purified antibody has been tested for use in ELISA (1: 7,500-1: 30,000) againstthe immunizing peptide and by Western blotting (1: 500-1: 2,000) against HeLa whole celllysates and nuclear extracts. Reactivity in other immunoassays is unknown. Antibodyactivity can be blocked using a control peptide. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.3 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.12 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
MAD2L2 is a component of the mitotic spindle assembly checkpoint that prevents the onset of anaphase until all chromosomes are properly aligned at the metaphase plate. MAD2L2 is a homolog of MAD2L1.Synonyms: MAD2B, Mitotic spindle assembly checkpoint protein MAD2B, REV7