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- 抗原 See all Glycogen Synthase (GYS) 抗体
- Glycogen Synthase (GYS)
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抗原表位
- pSer641
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适用
- 人, 小鼠
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Glycogen Synthase antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 序列
- A-Q-G-Y-R-Y-P-R-P-A-(pS)-V-P (aa 631-642)
- 特异性
- This affinity purified antibody is directed against human muscle glycogen synthase. The product was affinity purified from monospecific antiserum by Immunoaffinity purification. Antiserum was first purified against the phosphorylated form of the immunizing peptide. The resultant affinity purified antibody was then cross-adsorbed against the non-phosphorylated form of the immunizing peptide. This phospho specific polyclonal antibody is specific for phosphorylated pS640 of human muscle glycogen synthase. Reactivity with non-phosphorylated human muscle glycogen synthase is less than 1 % by ELISA. Cross reactivity with muscle glycogen synthase occurs in mouse tissue. Reactivity with muscle glycogen synthase from other sources has not been determined.
- 纯化方法
- Immunoaffinity chromatography.
- 免疫原
- Human Muscle Glycogen Synthase phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
- Top Product
- Discover our top product GYS Primary Antibody
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anti-Glycogen Synthase (GYS) (AA 611-660), (pSer645) antibody
GYS 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase (GYS) (AA 621-670) antibodyGYS 适用: 人, 小鼠, 大鼠 WB, ELISA 宿主: 兔 Polyclonal unconjugated
anti-Glycogen Synthase (GYS) (pSer641) antibodyGYS 适用: 人, 小鼠 WB, EIA, IP, IHC (p) 宿主: 兔 Polyclonal unconjugated
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- 应用备注
- This phospho specific polyclonal antibody was tested by Immunoblotting and ELISA. By ELISA the antibody was found to be reactive with the phosphorylated form of theimmunizing peptide and minimally reactive with the non-phosphorylated form of theimmunizing peptide. Immunoblotting will detect human and mouse muscle glycogensynthase. Although not tested, this antibody is likely functional in Immunohistochemistryand Immunoprecipitation. This product has been assayed against 0.1 g of phosphorylatedpeptide in a standard capture ELISA using TMB (3,3',5,5'-Tetramethylbenizidine) as asubstrate for 30 minutes at room temperature. A working dilution of 1: 30,000 to 1: 150,000is suggested for this product. Less than 0.2 % cross-reactivity was detected against thenon-phosphorylated form o f the immunizing peptide. Dilute the antibody 1: 1,000 for
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store undiluted at 2-8 °C one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Glycogen Synthase (GYS)
- 别名
- Glycogen Synthase (GYS 产品)
- 别名
- CG6940 antibody, Dmel\\CG6904 antibody, anon-WO0118547.108 antibody, glycogen synthase antibody, Glycogen synthase antibody, starch synthase antibody, glgA antibody, GlyS antibody, glgA-2 antibody, glgA-1 antibody, EAMY_RS33325 antibody, Hipma_0956 antibody, Tresu_1353 antibody, Spico_0935 antibody, Trebr_1511 antibody, Mahau_1987 antibody
- 背景
- Human muscle glycogen synthase (GS) is responsible for the biosynthesis of glycogen from phosphorylated glucose units. Mammalian liver and muscle contain GS consisting of four subunits with a total molecular weight of 360,000. GS is subject to regulation through both allosteric and covalent modification and occurs in two forms: the phosphorylated inactive form, and the dephosphorylated active form. GS is inactivated by the serine/threonine kinase called glycogen synthase kinase-3β that mainly functions to phosphorylate muscle glycogen synthase. This antibody is specific for the phosphorylated form of GS at S640. Phosphorylation of GS at S640 has been associated with Antiphospholipid Antibody Syndrome.Synonyms: GYS, GYS1, muscle Glycogen Synthase
- 基因ID
- 2997
- NCBI登录号
- NP_002094
- UniProt
- P13807
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