This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human and mouse Cullin 7. Cross reactivity with other human cullins may occur.
纯化方法
Delipidation and defibrination.
免疫原
Synthetic peptide corresponding to amino acids 1679-1698 of Human Cul7 (C-terminus) coupled coupled to KLH.
Western blot (1/500-1/1,000): This antibody reacts with human Cul7, An 191.2 kDa bandcorresponding to human Cul7 is detected, Most cell lines expressing Cul7 can be used as apositive control. Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein andalso protein from cell lysates (using HeLa or NIH-3T3 and others. This antibody alsoco-immunoprecipitates associated proteins in overexpressed CUL7 systems). ELISA: 1/2,000-1/10,000 Dilution. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 7 is a component of E3 ubiquitin ligase complexes, which mediate the ubiquitination and subsequent proteasomal degradation of target proteins. Cullin 7 seems to be involved proteosomal degradation of proteins involved in endothelial proliferation and/or differentiation. Cullin 7 is part of a SCF-like complex consisting of CUL7, RBX1, SKP1, FBXW8 and GLMN isoform 1. Cullin 7 interacts with a complex of SKP1 and FBXW8, but not with SKP1 alone.Synonyms: CUL-7, CUL7, KIAA0076