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Cullin 1 抗体 (C-Term)

Name: Cullin 1 抗体 (C-Term)
SKU: ABIN117947
Availability: OutOfStock

CUL1 适用: 人, 小鼠 WB, IP, IHC (p), EIA 宿主: 兔 Polyclonal unconjugated

Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). (Cullin 1 抗体 (C-Term))

1 image

产品编号 ABIN117947

发货至: 中国

Datasheet as PDF

抗原 See all Cullin 1 (CUL1) 抗体

Cullin 1 (CUL1)
抗原表位
17

15

8

6

3

3

2

2

2

2

1

1

1

1

1

1
AA 742-752, C-Term
适用
77

34

29

7

6

5

5

5

4

4

2

2

2

2

1

1
人, 小鼠
宿主
76

1
兔
Compatible Secondaries
克隆类型
68

9
多克隆
标记
39

5

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1
This Cullin 1 antibody is un-conjugated
应用范围
43

27

16

14

14

13

11

7

5

3

2

1

1

1

1
Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

序列

Human Cullin 1 (776 aa 89.6 kDa): 1 msstrsqnph glkqigldqi wddlragiqq vytrqsmaks rymelythvy nyctsvhqsn 61 qargagvpps kskkgqtpgg aqfvglelyk rlkeflknyl tnllkdgedl mdesvlkfyt 121 qqwedyrfss kvlngicayl nrhwvrrecd egrkgiyeiy slalvtwrdc lfrplnkqvt 181 navlklieke rngetintrl isgvvqsyve lglneddafa kgptltvyke sfesqfladt 241 erfytreste flqqnpvtey mkkaearlle eqrrvqvylh estqdelark ceqvliekhl 301 eifhtefqnl ldadknedlg rmynlvsriq dglgelkkll ethihnqgla aiekcgeaal 361 ndpkmyvqtv ldvhkkynal vmsafnndag fvaaldkacg rfinnnavtk maqssskspe 421 llarycdsll kkssknpeea eledtlnqvm vvfkyiedkd vfqkfyakml akrlvhqnsa 481 sddaeasmis klkqacgfey tsklqrmfqd igvskdlneq fkkhltnsep ldldfsiqvl 541 ssgswpfqqs ctfalpsele rsyqrftafy asrhsgrklt wlyqlskgel vtncfknryt 601 lqastfqmai llqynteday tvqqltdstq ikmdilaqvl qillkskllv ledenanvde 661 velkpdtlik lylgyknkkl rvninvpmkt eqkqeqetth knieedrkll iqaaivrimk 721 mrkvlkhqql lgevltqlss rfkprvpvik kcidilieke ylervdgekd tysyla

特异性

This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human Cullin 1. Cross reactivity is expected against mouse Cul1 based on sequence homology.

纯化方法

Delipidation and defibrination.

免疫原

Synthetic peptide corresponding to amino acids 742-752 of Human Cul1 (C-terminus) coupled to KLH.

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anti-Cullin 1 (CUL1) antibody
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anti-Cullin 1 (CUL1) antibody
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anti-Cullin 1 (CUL1) (AA 1-776) antibody
CUL1 适用: 人 WB, IF, PLA 宿主: 兔 Polyclonal unconjugated

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CUL1 适用: 人, 小鼠, 大鼠 WB, ELISA, IHC, IF, ICC 宿主: 兔 Polyclonal unconjugated

anti-Cullin 1 (CUL1) (C-Term) antibody
CUL1 适用: 人 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cullin 1 (CUL1) (C-Term) antibody
CUL1 适用: 人, 小鼠 WB, ELISA, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Cullin 1 (CUL1) (C-Term) antibody
CUL1 适用: 人 WB, ELISA, IHC, IP, IHC (p) 宿主: 小鼠 Monoclonal unconjugated

anti-Cullin 1 (CUL1) (C-Term) antibody
CUL1 适用: 人, 小鼠, 大鼠, 小鸡, Cow, 犬, 兔, Pig WB, IHC, IF, IC 宿主: 兔 Polyclonal unconjugated

应用备注

Western blot: This antibody reacts with human Cul1 (1/500-1/1,000, An 89.6 kDa bandcorresponding to human Cul1 is detected). Most cell lines expressing Cul1 can be used as apositive control. Immunoprecipitation: The antibody immunoprecipitates in vitro translated product andprotein from cell lysates (using HeLa or NIH-3T3). Do not IP in the presence of NP-40, butrather use 0.1 % SDS). ELISA (1/2,000-1/10,000).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
状态

Liquid

浓度

85.0 mg/mL (by Refractometry)

注意事项

Avoid repeated freezing and thawing.

储存条件

4 °C/-20 °C

储存方法

Store undiluted at 2-8 °C for one month or at -20 °C for longer.
抗原

Cullin 1 (CUL1)

别名

Cullin 1 (CUL1 产品)

别名

LOC100282292 antibody, Cullin-1 antibody, Cul1 antibody, zgc:55747 antibody, zgc:77500 antibody, wu:fc13a10 antibody, cullin-1 antibody, ATCUL1 antibody, AUXIN RESISTANT 6 antibody, AXR6 antibody, T10P11.26 antibody, T10P11_26 antibody, cullin 1 antibody, cul1 antibody, wu:fi43h07 antibody, wu:fj43a08 antibody, zgc:111899 antibody, zgc:55483 antibody, Cul-1 antibody, cullin 1 antibody, cullin-1 antibody, Cullin 1 antibody, Cullin-1 antibody, cullin 1a antibody, cullin 1 S homeolog antibody, cullin 1b antibody, CUL1 antibody, LOC100282292 antibody, cul1 antibody, ATEG_00491 antibody, EDI_072700 antibody, EDI_264400 antibody, SJAG_02591 antibody, BDBG_03785 antibody, Cul1 antibody, cul1a antibody, cul1.S antibody, cul-1 antibody, cul1b antibody

背景

Cullins assemble a potentially large number of ubiquitin ligases by binding to the RING protein ROC1 to catalyse polyubiquitination, as well as binding to various specificity factors to recruit substrates. Cullin 1 is an essential component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex, which mediates the ubiquitination of proteins involved in cell cycle progression, signal transduction and transcription. In the SCF complex, cul1 serves as a rigid scaffold that organizes the SKP1-F-box protein and RBX1 subunits. Cul1 may also contribute to catalysis through positioning of the substrate and the ubiquitin-conjugating enzyme. Cul1 is part of the SCF complex consisting of CUL1, RBX1, SKP1 and SKP2, where it interacts directly with SKP1, SKP2 and RBX1. Cul1 also interacts with RNF7 and is part of a complex with TIP120A/CAND1 and RBX1. The unneddylated form interacts with TIP120A/CAND1 and the interaction negatively regulates the association with SKP1 in the SCF complex.Synonyms: CUL-1, CUL1, Cullin-1

基因ID

8454

NCBI登录号

NP_003583

UniProt

Q13616

途径

Cell Division Cycle, Hedgehog Signaling, Mitotic G1-G1/S Phases, Regulation of Cell Size