This is an affinity purified antibody produced by Immunoaffinity chromatography using the immunizing peptide after immobilization to a solid phase. Reactivity occurs against human HR23A protein. Sequence homology as assessed by BLAST indicated 100 % homology for this protein from human and rabbit. Cross reactivity with HR23A protein from dog, mouse and rat may also occur as sequence homology varies by one or two amino acid residues in this sequence by BLAST analysis. Reactivity with HR23A protein from other sources is not known.
纯化方法
Immunoaffinity chromatography.
免疫原
This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding aa 122-135 of human HR23A protein.
This affinity purified antibody has been tested for use in ELISA (1: 4,000-1: 6,000) and byWestern blot (1: 500-1: 2,000). Specific conditions for reactivity should be optimized by the end user. Expect a bandapproximately 60 kDa in size corresponding to HR23A by Western blotting in theappropriate cell lysate or extract. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.2 mg/mL (by UV absorbance at 280 nm)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store vial at -20 °C prior to opening. Aliquot contents and freeze at -20 °C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
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背景
HR23A (also known as UV excision repair protein RAD23 homolog A) is one of two human homologs of Saccharomyces cerevisiae Rad23 (hHR23A and hHR23B), a protein involved in nucleotide excision repair (NER). This protein was shown to interact with, and elevate the nucleotide excision activity of 3-methyladenine-DNA glycosylase (MPG), which suggested a role in DNA damage recognition in base excision repair. This protein contains an N-terminal ubiquitin-like domain, which was reported to interact with 26S proteasome, as well as with ubiquitin protein ligase E6AP, and thus suggests that this protein may be involved in the ubiquitin mediated proteolytic pathway in cells.Synonyms: HR23A, UV excision repair protein RAD23 homolog A