Cyanine Dyes 抗体 (Biotin)
IF, EIA, FACS, IHC (fro)
宿主: 绵羊
Polyclonal
Biotin
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- 抗原
- Cyanine Dyes
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宿主
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绵羊
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克隆类型
- 多克隆
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标记
- Biotin
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应用范围
- Immunofluorescence (IF), Enzyme Immunoassay (EIA), Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 产品特性
- Molar Ratio: 10-20 BAC molecule per sheep IgG molecule.
- 纯化方法
- Immunoaffinity chromatography
- 免疫原
- A mixture of Cy3 and Cy5 conjugated KLH
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- 应用备注
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Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assay using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended dilutions: This product has been assayed against 1.0 μg of Cyanine IgG in astandard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS(2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes atroom temperature. A working dilution of 1: 8,000 to 1: 40,000 of the reconstitution concentration is suggestedfor this product.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
- 浓度
- 1.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % Sodium Azide as preservative and 10 mg/mL BSA as stabilzer, IgG and Protease free.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- Cyanine Dyes
- 物质类
- Chemical
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