PKA RIIalpha (pSer99) 抗体 (Alexa Fluor 647)
适用: 人
ICS
宿主: 小鼠
Monoclonal
I65-856-286
Alexa Fluor 647
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- 抗原
- PKA RIIalpha
- 抗原表位
- pSer99
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 标记
- Alexa Fluor 647
- 应用范围
- Intracellular Staining (ICS)
- 品牌
- BD Phosflow™
- 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 免疫原
- Phosphorylated Human PKA RIIalpha Peptide
- 克隆位点
- I65-856-286
- 亚型
- IgG1 kappa
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- 应用备注
- Either BD Cytofix™ fixation buffer or BD™ Phosflow Fix Buffer I may be used for cell fixation. Any of the three BD™ Phosflow permeabilization buffers may be used.
- 样本量
- 20 μL
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 储存方法
- The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed. Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze.
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- 抗原
- PKA RIIalpha
- 别名
- PKA RIIa
- 背景
- CAMP-dependent Protein Kinase (PKA) is composed of two distinct subunits: catalytic (C) and regulatory (R). Four regulatory subunits have been identified: RIalpha, RIbeta, RIIalpha, and RIIbeta. These subunits define type I and II PKAs. Following binding of cAMP, the regulatory subunits dissociate from the catalytic subunits, rendering the enzyme active. Type I and II holoenzymes have three potential C subunits (Calpha, Cbeta, or Cgamma). Type II PKA can be distinguished by autophosphorylation of the R subunits, while type I PKA binds Mg/ATP with high affinity. The cAMP-dependent autophosphorylation of the human RIIalpha subunit occurs at Serine 99 (S99) [Entrez Protein Accession #CAA33094]. Most cells express both type I and type II PKAs. Although the Ralpha isoforms are ubiquitously expressed, the Rbeta isoforms are predominantly found in nervous and adipose tissues. In addition to their enzyme regulatory activity, the RIIalpha and RIIbeta subunits determine the subcellular location of the holoenzymes via their interactions with specific intracellular anchoring proteins. The I65-856.286 antibody recognizes human PKA RIIalpha phosphorylated at S99.
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