The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
免疫原
Phosphorylated Human Btk Peptide
克隆位点
24a-BTK
亚型
IgG1 kappa
样本量
20 μL
限制
仅限研究用
状态
Liquid
缓冲液
Aqueous buffered solution containing BSA and ≤0.09 % sodium azide.
储存液
Sodium azide
注意事项
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
储存条件
4 °C
储存方法
Store undiluted at 4°C and protected from prolonged exposure to light. Do not freeze. The antibody was conjugated to Alexa Fluor® 647 under optimum conditions, and unreacted Alexa Fluor® 647 was removed.
Wilcox, Berg: "Itk phosphorylation sites are required for functional activity in primary T cells." in: The Journal of biological chemistry, Vol. 278, Issue 39, pp. 37112-21, (2003) (PubMed).
抗原
Btk and Itk
背景
Bruton's tyrosine kinase (Btk) is a nonreceptor tyrosine kinase whose function is critical for proper B cell development and signaling. The activity of Btk is regulated by Src mediated phosphorylation of the kinase domain at tyrosine 551 (Y551). This event induces Btk kinase activity and subsequent autophosphorylation at Y223. Phosphorylated Btk then associates with the cell membrane via the interaction of the PH domain with phosphatidylinositol 3, 4, 5-triphosphate. The Tec family kinase Itk plays a critical role in signal transduction downstream of the T cell antigen receptor and has been implicated in the activation of phospholipase C-gamma1 (PLCgamma1), a key regulator of calcium mobilization and extracellular signal-regulated kinase (ERK) activation. Itk is regulated by an activating transphosphorylation event in which Y511 in the kinase domain is phosphorylated by Lck. The 24a/BTK (Y551) monoclonal antibody recognizes the Y551-phosphorylated form of human Btk and the Y511 phosphorylated form of human Itk.