The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
过滤
0.2 μm filtered
内毒素水平
Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
免疫原
Mouse Fetal Liver
克隆位点
TER-119
亚型
IgG2b kappa
限制
仅限研究用
状态
Liquid
浓度
1.0 mg/mL
缓冲液
No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
储存液
Azide free
储存条件
4 °C
储存方法
Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Kina, Ikuta, Takayama, Wada, Majumdar, Weissman, Katsura: "The monoclonal antibody TER-119 recognizes a molecule associated with glycophorin A and specifically marks the late stages of murine erythroid lineage." in: British journal of haematology, Vol. 109, Issue 2, pp. 280-7, (2000) (PubMed).
Maraskovsky, Brasel, Teepe, Roux, Lyman, Shortman, McKenna: "Dramatic increase in the numbers of functionally mature dendritic cells in Flt3 ligand-treated mice: multiple dendritic cell subpopulations identified." in: The Journal of experimental medicine, Vol. 184, Issue 5, pp. 1953-62, (1996) (PubMed).
Ikuta, Kina, MacNeil, Uchida, Peault, Chien, Weissman: "A developmental switch in thymic lymphocyte maturation potential occurs at the level of hematopoietic stem cells." in: Cell, Vol. 62, Issue 5, pp. 863-74, (1990) (PubMed).
抗原
TER-119/Erythroid Cells
背景
The TER-119 antibody specifically binds to a 52 kDa molecule associated with glycophorin A on cells of the erythroid lineage in embryonic yolk sac, fetal liver, newborn liver, adult bone marrow, adult peripheral blood, and adult lymphoid organs. The TER-119 antigen is expressed on erythroid cells from pro-erythroblast through mature erythrocyte stages, but not on cells with BFU-E or CFU-E activities. The TER-119 epitope is not detected on hematopoietic stem cells, lymphoid cells, myeloid cells, or erythroleukemia lines. The TER-119 mAb is a component of the lineage cocktail used in studies of hematopoietic progenitors to detect, or deplete cells committed to the hematopoietic lineages. Synonyms: Lymphocyte antigen 76, Ly76, Ly-76, TER-119, Ter119