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CD25 抗体

Name: CD25 抗体
SKU: ABIN1176951
Availability: OutOfStock

This anti-CD25 antibody is a 大鼠单克隆 antibody detecting CD25 in FACS 和 BR. Suitable for 小鼠. This Primary Antibody has been cited in 13+ publications.

(13 references)

产品编号 ABIN1176951

发货至: 中国

Datasheet as PDF

Quick Overview for CD25 抗体 (ABIN1176951)

抗原

See all CD25 (IL2RA) 抗体

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

适用

小鼠

宿主

大鼠

克隆类型

单克隆

标记

This CD25 antibody is un-conjugated

应用范围

Flow Cytometry (FACS), Blocking Reagent (BR)

克隆位点

PC61

Discover our top product IL2RA Primary Antibody

品牌

BD Pharmingen™

纯化方法

The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

过滤

0.2 μm filtered

内毒素水平

Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.

免疫原

IL-2-dependent cytolytic mouse T-cell clone B6.1

亚型

IgG1 lambda
anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 201-268) antibody
IL2RA 适用: 人, 小鼠 FACS, WB, ELISA, IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
IL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, ICC 宿主: 小鼠 Monoclonal 1B5D12 unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-213) antibody
IL2RA 适用: 人 WB, ELISA, ICC, IHC (fro), IHC (p) 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 34-139) antibody
IL2RA 适用: 人, 小鼠, 大鼠, 猴 WB, IHC, ELISA, IF/ICC 宿主: 小鼠 Monoclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-245) antibody
IL2RA 适用: Pig WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) antibody
IL2RA 适用: 小鼠, 大鼠 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 42-183) antibody
IL2RA 适用: 人 IHC, StM 宿主: 小鼠 Monoclonal IL2RA-2395 unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) antibody
IL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 unconjugated

anti-Interleukin 2 Receptor, alpha (IL2RA) antibody (PerCP)
IL2RA 适用: 人 FACS 宿主: 小鼠 Monoclonal MEM-181 PerCP

anti-Interleukin 2 Receptor, alpha (IL2RA) (AA 22-235) antibody
IL2RA 适用: 小鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated

应用备注

Flow Cytometry: Investigators may also wish to consider using PE Rat Anti-Mouse CD25 (Cat. No. 553866) or Biotin Rat Anti-Mouse CD25 (Cat. No. 553069) coupled with PE Streptavidin (Cat. No. 554061) for samples expressing low levels of CD25.
Immunohistochemistry: Investigators are encouraged to consider using Biotin Rat Anti-Mouse CD25 (Cat. No. 550529) for the immunohistochemistry application.

限制

仅限研究用
状态

Liquid

浓度

1.0 mg/mL

缓冲液

No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.

储存液

Azide free

储存条件

4 °C

储存方法

Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
Read, Malmström, Powrie: "Cytotoxic T lymphocyte-associated antigen 4 plays an essential role in the function of CD25(+)CD4(+) regulatory cells that control intestinal inflammation." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 295-302, (2000) (PubMed).

Takahashi, Tagami, Yamazaki, Uede, Shimizu, Sakaguchi, Mak, Sakaguchi: "Immunologic self-tolerance maintained by CD25(+)CD4(+) regulatory T cells constitutively expressing cytotoxic T lymphocyte-associated antigen 4." in: The Journal of experimental medicine, Vol. 192, Issue 2, pp. 303-10, (2000) (PubMed).

Chen, Ma, Young, Alt: "IL-2 receptor alpha chain expression during early B lymphocyte differentiation." in: International immunology, Vol. 6, Issue 8, pp. 1265-8, (1995) (PubMed).

Rolink, Grawunder, Winkler, Karasuyama, Melchers: "IL-2 receptor alpha chain (CD25, TAC) expression defines a crucial stage in pre-B cell development." in: International immunology, Vol. 6, Issue 8, pp. 1257-64, (1995) (PubMed).

Godfrey, Zlotnik: "Control points in early T-cell development." in: Immunology today, Vol. 14, Issue 11, pp. 547-53, (1994) (PubMed).

Taniguchi, Minami: "The IL-2/IL-2 receptor system: a current overview." in: Cell, Vol. 73, Issue 1, pp. 5-8, (1993) (PubMed).

Garni-Wagner, Witte, Tutt, Kuziel, Tucker, Bennett, Kumar: "Natural killer cells in the thymus. Studies in mice with severe combined immune deficiency." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 144, Issue 3, pp. 796-803, (1990) (PubMed).

Pollard, Lipscomb: "Characterization of murine lung dendritic cells: similarities to Langerhans cells and thymic dendritic cells." in: The Journal of experimental medicine, Vol. 172, Issue 1, pp. 159-67, (1990) (PubMed).

Crowley, Inaba, Witmer-Pack, Steinman: "The cell surface of mouse dendritic cells: FACS analyses of dendritic cells from different tissues including thymus." in: Cellular immunology, Vol. 118, Issue 1, pp. 108-25, (1989) (PubMed).

Moreau, Nabholz, Diamantstein, Malek, Shevach, Thèze: "Monoclonal antibodies identify three epitope clusters on the mouse p55 subunit of the interleukin 2 receptor: relationship to the interleukin 2-binding site." in: European journal of immunology, Vol. 17, Issue 7, pp. 929-35, (1987) (PubMed).

Ceredig, Lowenthal, Nabholz, MacDonald: "Expression of interleukin-2 receptors as a differentiation marker on intrathymic stem cells." in: Nature, Vol. 314, Issue 6006, pp. 98-100, (1985) (PubMed).

Lowenthal, Corthésy, Tougne, Lees, MacDonald, Nabholz: "High and low affinity IL 2 receptors: analysis by IL 2 dissociation rate and reactivity with monoclonal anti-receptor antibody PC61." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 135, Issue 6, pp. 3988-94, (1985) (PubMed).

Lowenthal, Zubler, Nabholz, MacDonald: "Similarities between interleukin-2 receptor number and affinity on activated B and T lymphocytes." in: Nature, Vol. 315, Issue 6021, pp. 669-72, (1985) (PubMed).
抗原

CD25 (IL2RA) (Interleukin 2 Receptor, alpha (IL2RA))

别名

CD25

背景

The PC61 antibody reacts with CD25, the low-affinity IL-2 Receptor alpha chain (IL-2Ralpha, p55) expressed on activated T and B lymphocytes from all mouse strains tested. IL-2Ralpha by itself is not a signaling receptor. However, it can combine with IL-2 Receptor beta (CD122) and gammac (CD132) chains to c form high-affinity, signaling receptor complexes for IL-2. Resting T and B lymphocytes and resting and activated NK cells do not express IL-2Ralpha. CD25 is transiently expressed at a low level during normal B-cell development in the bone marrow on the CD45R/B220low TdT- sIg- Pre-B/Pre-B-II and CD45R/B220low TdT- sIgM+ sIgD- immature B stages, but not on the CD45R/B220low TdT+ sIg- Pro-B/Pre-B-I stage nor on CD45R/B220high TdT- sIgM+ sIgD+ mature B cells. It is expressed at a higher level during a very early stage of T-cell development in fetal and adult thymus. Peripheral CD25+CD4+ lymphocytes called regulatory T (Treg) cells are involved in the maintenance of self-tolerance. It has also been reported that dendritic cells express CD25, recognized by mAb 7D4 (Cat. No. 553068). The PC61 antibody recognizes an epitope of CD25 which is distinct from the IL-2 binding site and from those recognized by mAbs 3C7 (Cat. No. 557364) and 7D4 (Cat. No. 553068). It blocks binding of IL-2 to CD25, presumably by inducing a conformational change in CD25. Use of this product can fall under one or more claims of the following patents licensed to Becton, Dickinson and Company: US Patent Nos. 5,445,939, 5,656,446, 5,843,689, European Patent No. 319,543, Canadian Patent No. 1,296,622, Australian Patent No. 615,880, and Japanese Patent No. 2,769,156.
Synonyms: Interleukin-2 receptor alpha chain, IL-2RA, IL-2Ralpha, Il2ra, IL-2R p55

途径

JAK/STAT Signaling, Growth Factor Binding, Activated T Cell Proliferation