(6 references)-
- 抗原 See all IFN alpha 2b products
- IFN alpha 2b
- 适用
- 人
- 宿主
- 小鼠
- 克隆类型
- 单克隆
- 应用范围
- Intracellular Staining (ICS), Neutralization (Neut)
- 品牌
- BD Pharmingen™
- 产品特性
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Neutralization Activity: This antibody has been reported to be useful for the neutralization of recombinant human IFN-alpha. Neutralization activity may be measured using MTT dye conversion in a IFN-alpha mediated EMC viral resistance assay using A549 as indicator cells.
50% Neutralization (ND50) at 0.3 - 3 µg/mL
>95% Neutralization at 10 - 50 myg/ml - 纯化方法
- The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.
- 过滤
- 0.2 μm filtered
- 内毒素水平
- Endotoxin level is ≤ 0.01 EU/μg (≤ 0.001 ng/μg) of protein as determined by the LAL assay.
- 免疫原
- Human IFN-alpha2b
- 克隆位点
- 7N4-1
- 亚型
- IgG1 kappa
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- 应用备注
- Flow cytometry: Conjugated formats of this clone may be used for immunofluorescent staining, such as PE Mouse Anti-Human IFN-alpha[2b] (Cat. No. 560097) or Alexa Fluor® 647 Mouse Anti-Human IFN-alpha[2b] (Cat. No. 560088).
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 1.0 mg/mL
- 缓冲液
- No azide/low endotoxin: Aqueous buffered solution containing no preservative, 0.2μm sterile filtered.
- 储存液
- Azide free
- 储存条件
- 4 °C
- 储存方法
- Store undiluted at 4°C. This preparation contains no preservatives, thus it should be handled under aseptic conditions.
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: "The human interferon alpha species and receptors." in: Biopolymers, Vol. 55, Issue 4, pp. 254-87, (2001) (PubMed).
: "The nature of the principal type 1 interferon-producing cells in human blood." in: Science (New York, N.Y.), Vol. 284, Issue 5421, pp. 1835-7, (1999) (PubMed).
: "Interferon-alpha 2 produced by normal human leukocytes is predominantly interferon-alpha 2b." in: Journal of interferon research, Vol. 14, Issue 6, pp. 325-32, (1995) (PubMed).
: "Immunochemical mapping of alpha-2 interferon." in: Biochemistry, Vol. 24, Issue 15, pp. 4131-41, (1985) (PubMed).
: "The human interferons--from protein purification and sequence to cloning and expression in bacteria: before, between, and beyond." in: Archives of biochemistry and biophysics, Vol. 221, Issue 1, pp. 1-37, (1983) (PubMed).
: "Rapid, quantitative, semiautomated assay for virus-induced and immune human interferons." in: Journal of clinical microbiology, Vol. 12, Issue 3, pp. 433-8, (1981) (PubMed).
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: "The human interferon alpha species and receptors." in: Biopolymers, Vol. 55, Issue 4, pp. 254-87, (2001) (PubMed).
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- 抗原
- IFN alpha 2b
- 别名
- IFN-alpha 2b (IFN alpha 2b 产品)
- 别名
- IFN-alphaA antibody, IFNA antibody, IFNA2B antibody, INFA2 antibody, interferon alpha 2 antibody, IFNA2 antibody
- 背景
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The 7N4-1 antibody reacts with human IFN-alpha2b and to a lesser extent with IFN-alpha7. It does not react with IFN-alpha1 nor IFN-alpha4. IFN-alpha2b is one of the three variants of IFN-alpha2 that have been isolated from human cell lines. IFN-alpha2b is the variant predominantly produced by human leukocytes. Human IFN-alpha2b belongs to the IFN-alpha class of proteins also known as leukocyte interferons. IFN-alpha comprises a family of related but distinct proteins with molecular weights ranging from 16-27 kDa with antiviral, antiproliferative and immunomodulatory activities. The IFN-alpha family is composed from as many as 14 different genes. The immunogen used to generate the 7N4-1 hybridoma was E. coli-expressed recombinant human IFN-alpha2b. This is a neutralizing antibody.
Synonyms: IFNa, IFNalpha
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