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- 抗原
- Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))
- 适用
- Saccharomyces cerevisiae
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This Hub1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA)
- 特异性
- Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum.
- 纯化方法
- A multi-step process which includes Delipidation, Salt Fractionation and Ion exchange chromatography followed by extensive dialysis against the buffer stated below.
- 免疫原
- This purified antibody was prepared from rabbit serum after repeated immunizations with recombinant yeast Hub1 protein.
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anti-Ubiquitin-Like Protein Modifier Hub1 (Hub1) antibody
Hub1 适用: Saccharomyces cerevisiae WB, ELISA, IP, IHC 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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This purified polyclonal antibody reacts with yeast Hub1 by Western blot and ELISA. This antibody using the specified conditions may recognize other prominent intrinsicbands (UBLs or conjugates).
Other intrinsic bands are readily detectable at lower dilutions. A 9.7 kDa band corresponding to yeast Hub1 is detected. Most yeast cell lysates can be used as a positive control without induction or stimulation. Recommended Dilutions: ELISA: 1/1,000-1/5,000. Western Blot: 1/500. Although not tested, this antibody is likely functional in Immunohistochemistry andImmunoprecipitation. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 0.1 mL of deionized water or equivalent.
- 浓度
- 5.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % (w/v) Sodium Azide as preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- Hub1 (Ubiquitin-Like Protein Modifier Hub1 (Hub1))
- 别名
- HUB1
- 背景
- Ubiquitin-like proteins fall into two classes: the first class, ubiquitin-like modifiers (UBLs) function as modifiers in a manner analogous to that of ubiquitin. Examples of UBLs are SUMO, Rub1 (also called Nedd8), Apg8 and Apg12. Proteins of the second class include parkin, RAD23 and DSK2, are designated ubiquitin-domain proteins (UDPs). These proteins contain domains that are related to ubiquitin but are otherwise unrelated to each other. In contrast to UBLs, UDPs are not conjugated to other proteins. Hub1 ("Homologous to UBiquitin") may function as a modifier (see figure 2) but its role is unclear because it lacks the double glycine motif characteristic for ubiquitin and ubiquitin-like modifiers. Recently cell polarity factors Sph1 and Hbt1 have been identified as in vivo targets of Hub1 conjugation. HUB1 has close homologs in other species including humans. The human homologs is called Ubl5.Synonyms: Ubiquitin-like modifier HUB1, YNR032C-A
- 基因ID
- 855767
- NCBI登录号
- NP_014430
- UniProt
- Q6Q546
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