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His Tag 抗体

Name: His Tag 抗体
SKU: ABIN116941
Availability: OutOfStock

WB, IHC (fro), EIA 宿主: 兔 Polyclonal unconjugated

Anti-6X His epitope tag polyclonal antibody detects His-tagged recombinant proteins by western blot. (His Tag 抗体)

1 image

产品编号 ABIN116941

发货至: 中国

Datasheet as PDF

抗原 See all His Tag 抗体

His Tag

适用

请咨询
宿主
93

60

11

9

5

4

1

1
兔
Compatible Secondaries
克隆类型
95

76

10
多克隆
标记
79

21

14

13

5

4

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

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This His Tag antibody is un-conjugated
应用范围
143

100

39

27

25

19

15

14

14

13

13

11

7

5

5

4

4

4

3

3

2

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Enzyme Immunoassay (EIA)

序列

HHHHHH

纯化方法

Affinity Chromatography.

免疫原

6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide

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WB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal HIS-H8 unconjugated

anti-His Tag antibody (DyLight 649)
WB, ELISA, DB, FM, FLISA 宿主: 小鼠 Monoclonal 33D10-D2-G8 DyLight 649

应用备注

Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. This antibody has been tested by ELISA and western blotting against both the immunizingpeptide and His-containing recombinant proteins. Although not tested, this antibody islikely functional for Immunoprecipitation and Immunocytochemistry. Recommended Dilutions: ELISA: 1/10,000-1/40,000. Western blot: 1/1,000-1/3,000. Immunohistochemistry: 1/500-1/2,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

限制

仅限研究用
浓度

1.0 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, containing 0.01 % (w/v) Sodium Azide as preservative.

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

Dilute only prior to immediate use. Avoid cycles of freezing and thawing.

储存条件

-20 °C

储存方法

Store vial at -20 °C or below prior to opening. Aliquot contents and freeze at -20 °C or below.
抗原

His Tag

别名

6xHistidine Epitope Tag (His Tag 产品)

物质类

Tag

背景

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.