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- 抗原 See all DYKDDDDK Tag products
- DYKDDDDK Tag
- 适用
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This DYKDDDDK Tag antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 序列
- DYKDDDDK
- 纯化方法
- Affinity purification of this polyclonal antibody results in very low background levels in assays and low cross-reactivity with other cellular proteins.
- 免疫原
- This antibody was purified from whole rabbit serum prepared by repeated immunizations with the DYKDDDDK epitope tag peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH using maleimide. Residues of glycine and cysteine were added to the carboxy terminal end to facilitate coupling.
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anti-DYKDDDDK Tag antibody
WB, ELISA, IP 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, IP, IF 宿主: 小鼠 Monoclonal 2C5 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IF, FACS 宿主: 兔 Polyclonal unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP, DB, ISt 宿主: 小鼠 Monoclonal FG4R unconjugated
anti-DYKDDDDK Tag antibodyD 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibody (HRP)WB, ELISA, IP 宿主: 兔 Polyclonal HRP
anti-DYKDDDDK Tag antibodyIP 宿主: 小鼠 Monoclonal 29E4-G7 unconjugated
anti-DYKDDDDK Tag antibodyWB, ELISA, IP 宿主: 小鼠 Monoclonal 5A8E5 unconjugated
anti-DYKDDDDK Tag antibody适用: 标记 WB, ELISA, IP, FACS, IHC, ICC 宿主: 小鼠 Monoclonal 2F11G1 unconjugated
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- 应用备注
- This antibody is optimally suited for monitoring the expression of DYKDDDDK-taggedfusion proteins. As such, this antibody can be used to identify fusion proteins containingthe DYKDDDDK epitope. The antibody recognizes the epitope tag fused to either the aminoorcarboxy termini of targeted proteins. This antibody has been tested by ELISA andwestern blotting against both the immunizing peptide and DYKDDDDK containingrecombinant proteins. Although not tested, this antibody is likely functional for
- 限制
- 仅限研究用
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- 浓度
- 1.04 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 0.01 % Sodium Azide
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- DYKDDDDK Tag
- 别名
- DYKDDDDK Epitope Tag (DYKDDDDK Tag 产品)
- 物质类
- Tag
- 背景
- Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means. This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein. The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures. Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. Now the most commonly used hydrophilic octapeptide is DYKDDDDK.
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