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Recombinant eGFP 抗体

eGFP 适用: Aequorea victoria ELISA, IP, ICC 宿主: Human Monoclonal G3 unconjugated Recombinant Antibody
产品编号 ABIN1169226
发货至: 中国
  • 抗原 See all eGFP products
    eGFP (Enhanced Green Fluorescent Protein (eGFP))
    抗体类型
    Recombinant Antibody
    适用
    • 11
    • 3
    • 3
    • 3
    • 2
    • 2
    • 1
    Aequorea victoria
    宿主
    • 16
    • 5
    • 1
    Human
    克隆类型
    • 16
    • 6
    单克隆
    标记
    • 8
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This eGFP antibody is un-conjugated
    应用范围
    • 21
    • 13
    • 7
    • 3
    • 3
    • 2
    • 1
    • 1
    ELISA, Immunoprecipitation (IP), Immunocytochemistry (ICC)
    特异性
    Recognizes EGFP, Enhanced Cyan Fluorescent Protein (ECFP) and Enhanced Yellow Fluorescent Protein (EYFP).
    纯化方法
    Purified from HEK 293 cell culture supernatant.
    纯度
    >95 % (SDS-PAGE)
    免疫原
    Enhanced Green Fluorescent Proteins (EGFP).
    克隆位点
    G3
    亚型
    IgG2
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    说明

    anti-EGFP, monoclonal antibody (recombinant) (G3) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    In PBS containing 10 % glycerol and 0.02 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
    有效期
    12 months
  • Moutel, Vielemeyer, Jin, Divoux, Benaroch, Perez: "Fully in vitro selection of recombinant antibodies." in: Biotechnology journal, Vol. 4, Issue 1, pp. 38-43, (2009) (PubMed).

  • 抗原
    eGFP (Enhanced Green Fluorescent Protein (eGFP))
    别名
    EGFP (eGFP 产品)
    背景
    Anti-EGFP, monoclonal antibody (recombinant) (G3) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.
    UniProt
    P42212
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