Polyglutamylation 抗体 (Biotin)
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- 抗原
- Polyglutamylation
- 适用
- 所有种类
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- Biotin
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应用范围
- Immunocytochemistry (ICC), Immunoprecipitation (IP), Western Blotting (WB)
- 特异性
- Recognizes the posttranslational modification (poly)glutamylation on proteins. Reacts with polyglutamylated alpha- and beta-tubulin.
- 交叉反应
- 所有种类
- 纯化方法
- Purified from concentrated hybridoma tissue culture supernatant.
- 纯度
- >95 % (SDS-PAGE)
- 免疫原
- Octapeptide EGEGE*EEG, modified by the addition of two glutamyl units onto the fifth E (indicated by an asterisk).
- 克隆位点
- GT335
- 亚型
- IgG1
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anti-Polyglutamylation antibody
适用: 所有种类 ICC, IP, WB, IEM 宿主: 小鼠 Monoclonal GT335 unconjugated
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- 应用备注
- Optimal working dilution should be determined by the investigator.
- 说明
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Recognizes most forms of polyglutamylated tubulin (alpha- and beta-tubulin), independent of the length of the glutamate side chains. No specificity to particular tubulin isoforms nor to tubulin from particular species are observed. Detects also other (poly)glutamylated proteins. Since no consensus modification site is known for protein (poly)glutamylation, the detection is not sequence-specific. However, an acidic environment of the modification site is required. The use of the antibody at too high concentrations obscures its specificity in immunofluorescence.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- Lot specific
- 缓冲液
- In PBS containing 0.02 % sodium azide.
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C,-20 °C
- 储存方法
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Short Term Storage: +4°C
Long Term Storage: -20°C
Stable for at least 1 year after receipt when stored at -20°C. - 有效期
- 12 months
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- 抗原
- Polyglutamylation
- 背景
- Polyglutamylation is a post-translational modification in which glutamate side chains of variable lengths are formed on the modified protein. It is evolutionarily conserved and the most prominent substrate is tubulin, the microtubule (MT) building block. Polyglutamylation has been proposed to be involved in the functional adaptation of MTs, as it occurs within the carboxy-terminal tubulin tails that participate directly in the binding of many structural and motor MT-associated proteins. The recent identification of new substrates of polyglutamylation indicates that this post-translational modification could be a potential regulator of diverse cellular processes and be involved in cell cycle and cell proliferation.
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