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Recombinant Golgin B1 (GOLGB1) 抗体

GOLGB1 适用: 人, 小鼠 ICC 宿主: Human Monoclonal TA10 unconjugated Recombinant Antibody
产品编号 ABIN1169059
发货至: 中国
  • 抗原 See all Golgin B1 (GOLGB1) 抗体
    Golgin B1 (GOLGB1)
    抗体类型
    Recombinant Antibody
    适用
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    人, 小鼠
    宿主
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    Human
    克隆类型
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    单克隆
    标记
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    非结合性
    应用范围
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    Immunocytochemistry (ICC)
    特异性
    Recognizes human and mouse giantin.
    交叉反应
    人, 小鼠
    纯化方法
    Purified from HEK 293 cell culture supernatant.
    纯度
    >95 % (SDS-PAGE)
    免疫原
    Human recombinant giantin.
    克隆位点
    TA10
    亚型
    IgG2
    Top Product
    Discover our top product GOLGB1 Primary Antibody
  • 应用备注
    Optimal working dilution should be determined by the investigator.
    说明

    anti-Giantin, monoclonal antibody (recombinant) (TA10) is composed of human variable regions (VH and VL) (lambda-chain) of immunoglobulin fused to the human lgG2 Fc domain. anti-Giantin, monoclonal antibody (recombinant) (TA10) is an antibody developed by antibody phage display technology using a human naive antibody gene library. These libraries consist of scFv (single chain fragment variable) composed of VH (variable domain of the human immunoglobulin heavy chain) and VL (variable domain of the human immunoglobulin light chain) connected by a polypeptide linker. The antibody fragments are displayed on the surface of filamentous bacteriophage (M13). This scFv was selected by affinity selection on antigen in a process termed panning. Multiple rounds of panning are performed to enrich for antigen-specific scFv-phage. Monoclonal antibodies are subsequently identified by screening after each round of selection. The selected monoclonal scFv is cloned into an appropriate vector containing a Fc portion of interest and then produced in mammalian cells to generate an IgG like scFv-Fc fusion protein.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    Lot specific
    缓冲液
    In PBS containing 10 % glycerol and 0.02 % sodium azide.
    储存液
    Sodium azide
    注意事项
    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    储存条件
    4 °C,-20 °C
    储存方法
    Short Term Storage: +4°C
    Long Term Storage: -20°C
    Stable for at least 1 month after receipt when stored at +4°C. Stable for at least 1 year after receipt when stored at -20°C.
    有效期
    12 months
  • Vielemeyer, Nizak, Jimenez, Echard, Goud, Camonis, Rain, Perez: "Characterization of single chain antibody targets through yeast two hybrid." in: BMC biotechnology, Vol. 10, pp. 59, (2010) (PubMed).

    Nizak, Martin-Lluesma, Moutel, Roux, Kreis, Goud, Perez: "Recombinant antibodies against subcellular fractions used to track endogenous Golgi protein dynamics in vivo." in: Traffic (Copenhagen, Denmark), Vol. 4, Issue 11, pp. 739-53, (2003) (PubMed).

  • 抗原
    Golgin B1 (GOLGB1)
    别名
    Giantin (GOLGB1 产品)
    别名
    gcp antibody, golim1 antibody, giantin antibody, 4930428L02Rik antibody, 628101 antibody, 6330407A06Rik antibody, AU042952 antibody, C130074L01Rik antibody, F730017E11Rik antibody, Gm6840 antibody, mKIAA4151 antibody, GCP antibody, GCP372 antibody, GOLIM1 antibody, golgin B1 antibody, golgin B1 S homeolog antibody, golgi autoantigen, golgin subfamily b, macrogolgin 1 antibody, GOLGB1 antibody, golgb1.S antibody, Golgb1 antibody
    背景
    Giantin, the largest Golgi membrane protein, is C-terminally anchored to cis- and medial-Golgi membranes and forms a long ~250nm coiled-coil rod that directly interacts with microtubules in a cell-cycle regulated way. It is an excellent marker for mammalian golgi complex. It is also an antigen in chronic rheumatoid arthritis and in the autoimmune disease Sjoegren syndrome.
    UniProt
    Q14789
    途径
    SARS-CoV-2 Protein Interactome
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