Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)
特异性
Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Ribonuclease A [Bovine Pancreas]. Cross reactivity against Ribonuclease A from other tissues and species may occur but have not been specifically determined.
纯化方法
Prepared from monospecific antiserum by a delipidation and defibrination.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended dilutions: ELISA: 1/30,000-1/150,000. Western blot: 1/3,000-1/15,000. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
溶解方式
Restore with 2.0 mL of deionized water (or equivalent).
浓度
90.0 mg/mL (by Refractometry)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with no preservatives.
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing. Dilute only prior to immediate use
储存条件
4 °C/-20 °C
储存方法
Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
抗原
RNASE1
(Ribonuclease, RNase A Family, 1 (Pancreatic) (RNASE1))