HRP 抗体 (Biotin)
HRP
适用: Horseradish
WB, EIA, IHC (fro), IHC (p)
宿主: 兔
Polyclonal
Biotin
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- 抗原 See all HRP products
- HRP (Horseradish Peroxidase (HRP))
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适用
- Horseradish
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HRP antibody is conjugated to Biotin
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 产品特性
- Molar Ratio: 10-20 BAC molecules per Rabbit IgG molecule.
- 纯化方法
- Multi-step process.
- 免疫原
- Peroxidase from Horseradish.
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anti-Horseradish Peroxidase (HRP) antibody
HRP 适用: Horseradish WB, ELISA, ICC 宿主: 小鼠 Monoclonal HP-03 unconjugated
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- 应用备注
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Suitable for Immunoblotting, ELISA, Immunohistochemistry, Immunomicroscopy as well asother antibody based assays using streptavidin or avidin conjugates requiring lot-to-lotconsistency. Recommended Dilutions: ELISA: 1/20,000-1/100,000. Western blot: 1/2,000-1/10,000. Immunohistochemistry: 1/1,000-1/5,000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore with 1.0 mL of deionized water (or equivalent).
- 浓度
- 10.0 mg/mL (by UV absorbance at 280 nm)
- 缓冲液
- 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % (w/v) Sodium Azide as preservative and 10 mg/mL BSA (IgG and Protease free) as stabiliser.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. For extended storage add glycerol to 50% and then aliquot contents and freeze at -20 °C or below. Centrifuge product if not completely clear after standing at room temperature. This antibody is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- HRP (Horseradish Peroxidase (HRP))
- 别名
- Horseradish Peroxidase / HRP (HRP 产品)
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