HRP 抗体
HRP
适用: Horseradish
WB, IP, EIA, IHC (fro), IHC (p)
宿主: 兔
Polyclonal
unconjugated
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- 抗原 See all HRP products
- HRP (Horseradish Peroxidase (HRP))
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适用
- Horseradish
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This HRP antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunoprecipitation (IP), Enzyme Immunoassay (EIA), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 纯化方法
- Prepared from monospecific antiserum by a Delipidation and Defibrination
- 免疫原
- Peroxidase from Horseradish.
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anti-Horseradish Peroxidase (HRP) antibody
HRP 适用: Horseradish WB, ELISA, ICC 宿主: 小鼠 Monoclonal HP-03 unconjugated
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- 应用备注
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Suitable for Immunoblotting (Western or Dot blot, 1/2,000-1/10,000), ELISA(1/20,000-1/100,000), Immunoprecipitation, Immunohistochemistry (1/1,000-1/5,000)and most Immunological methods requiring high titer and specificity.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore with 2.0 mL of deionized water (or equivalent).
- 浓度
- 90.0 mg/mL (by Refractometry)
- 缓冲液
- 0.01 M Sodium Phosphate, 0.11 M Sodium Chloride, pH 7.2, None, None
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use. . Dilute only prior to immediate use
- 储存条件
- 4 °C
- 储存方法
- Store vial at 2-8 °C prior to restoration. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.
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- 抗原
- HRP (Horseradish Peroxidase (HRP))
- 别名
- Horseradish Peroxidase / HRP (HRP 产品)
- 背景
- Horseradish Peroxidase (HRP) is an enzyme commonly used as an indicator for chemical reactions which produce peroxide. The enzyme is routinely conjugated to antibodies for use in enzyme-based immunoassay systems. HRP functions in the removal of H2O2 (hydrogen peroxide), oxidation of toxic reductants, biosynthesis and degradation of lignin, suberization, auxin catabolism, response to environmental stresses such as wounding, pathogen attack and oxidative stress. These functions might be dependent on each isozyme/isoform in each plant tissue.
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