Assay by immunoelectrophoresis resulted in a single precipitin arc against purified and partially purified Esterase [Porcine Liver]. Cross reactivity against Esterase from other tissues and species may occur but have not been specifically determined.
纯化方法
Prepared from monospecific antiserum by a delipidation and defibrination.
Suitable for Immunoblotting (Western or Dot blot), ELISA, Immunoprecipitation and mostimmunological methods requiring high titer and specificity. Recommended Dilutions: This product has been assayed against 1.0 μg of Esterase[Porcine Liver] in a standard sandwich ELISA using Peroxidase conjugated Affinity Purifiedanti-Rabbit IgG [H&L] (Goat) and ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonicacid]) as a substrate for 30 minutes at room temperature. A working dilution of 1: 8,000 to1: 40,000 of the reconstitution concentration is suggested for this product. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
状态
Liquid
溶解方式
Restore with 2.0 mL of deionized water (or equivalent).
浓度
85.0 mg/mL (by Refractometry)
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01 % sodium azide as preservative., None
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Dilute only prior to immediate use. Avoid cycles of freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store vial at -20 °C prior to opening. For extended storage aliquot contents and freeze at or below -20 °C. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8 °C as an undiluted liquid.