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Collagen IV 抗体

Name: Collagen IV 抗体
SKU: ABIN116560
Availability: OutOfStock

COL4 适用: 人, 哺乳动物 WB, IHC (fro), IHC (p), IP, EIA 宿主: 兔 Polyclonal unconjugated

(3 references)

产品编号 ABIN116560

发货至: 中国

Datasheet as PDF

抗原 See all Collagen IV (COL4) 抗体

Collagen IV (COL4) (Collagen, Type IV (COL4))
适用
97

49

41

22

16

11

5

2
人, 哺乳动物
宿主
62

29

12
兔
Compatible Secondaries
克隆类型
73

30
多克隆
标记
47

10

7

6

3

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1
This Collagen IV antibody is un-conjugated
应用范围
63

46

32

31

30

26

26

24

14

9

9

8

3

2

2

1

1

1

1

1
Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Enzyme Immunoassay (EIA)

纯化方法

Immunoaffinity Chromatography.

免疫原

Collagen type IV purified from Human and Bovine placenta.

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anti-Collagen, Type IV (COL4) antibody
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anti-Collagen, Type IV (COL4) (AA 1571-1669) antibody
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anti-Collagen, Type IV (COL4) (AA 1401-1500) antibody
COL4 适用: 人, 小鼠, 大鼠, 兔 WB, ELISA, IHC (fro), IHC (p), FACS, IF (p), IF (cc) 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人 ELISA, IHC, IHC (fro), IHC (p), IF 宿主: 兔 Polyclonal unconjugated

anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人 IHC 宿主: 兔 Monoclonal MSVA-704R unconjugated Recombinant Antibody

anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人 ELISA 宿主: 山羊 Polyclonal unconjugated

anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人, 大鼠, Pig, Cow WB, IHC, IHC (fro), IHC (p), ICC 宿主: 小鼠 Monoclonal LP46 unconjugated

anti-Collagen, Type IV (COL4) antibody
COL4 适用: 人 WB, IHC, IHC (fro), ICC 宿主: 小鼠 Monoclonal 1043 unconjugated

anti-Collagen, Type IV (COL4) antibody (FITC)
COL4 适用: 人 ELISA 宿主: 小鼠 Monoclonal 2F11 FITC

anti-Collagen, Type IV (COL4) antibody (Biotin)
COL4 适用: 人, Cow, 哺乳动物 WB, ELISA, IHC, IP 宿主: 兔 Polyclonal Biotin

应用备注

Anti-Collagen antibodies have been used for indirect trapping ELISA for quantitation ofantigen in serum using a standard curve, for Immunoprecipitation and for native(non-denaturing, non-dissociating) PAGE and Western blotting for highly sensitivequalitative analysis. Recommended Dilutions: ELISA 1/5,000-1/20,000. Western blot: 1/500-1/5,000. Immunoprecipitation: 1/100. Immunohistochemistry: 1/50-1/200 (prepare tissues as frozen tissue sections). Note: This product was assayed by Immunoblot and found to be reactive against CollagenIV at a dilution of 1/5,000-1/10,000. This product was also assayed against 1.0 μg of Collagen IV in a standard sandwich ELISAusing Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) (Cat#R1364HRP)and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1/4,000-1/8,000 of the stock

限制

仅限研究用
浓度

1.0 mg/mL (by UV absorbance at 280 nm)

缓冲液

0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0, 0.01 % (w/v) Sodium Azide, None

储存液

Sodium azide

注意事项

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

注意事项

This product is photosensitive and should be protected from light

储存条件

4 °C

储存方法

Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
Rodriguez-Teja, Gronau, Breit, Zhang, Minamidate, Caley, McCarthy, Cox, Erler, Gaughan, Darby, Robson, Mauri, Waxman, Sturge: "AGE-modified basement membrane cooperates with Endo180 to promote epithelial cell invasiveness and decrease prostate cancer survival." in: The Journal of pathology, Vol. 235, Issue 4, pp. 581-92, (2015) (PubMed).

Behrens, Villone, Koch, Brunner, Sorokin, Robenek, Bruckner-Tuderman, Bruckner, Hansen: "The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens." in: The Journal of biological chemistry, Vol. 287, Issue 22, pp. 18700-9, (2012) (PubMed).

Sasse, Malan, Fleischmann, Roell, Gustafsson, Bostani, Fan, Kolbe, Breitbach, Addicks, Welz, Brem, Hescheler, Aszodi, Costell, Bloch, Fleischmann: "Perlecan is critical for heart stability." in: Cardiovascular research, Vol. 80, Issue 3, pp. 435-44, (2009) (PubMed).
抗原

Collagen IV (COL4) (Collagen, Type IV (COL4))

别名

Collagen Type IV (COL4 产品)

别名

collagen, type IV antibody, Let-2 antibody

背景

Collagens are highly conserved throughout evolution and are characterized by an uninterrupted "Glycine-X-Y" triplet repeat that is a necessary part of the triple helical structure. For these reasons it is often extremely difficult to generate antibodies with specificities to collagens. The development of type specific antibodies is dependent on NON-DENATURED three-dimensional epitopes. Collagens were extensively purified for immunization from human and bovine placenta and cartilage by limited pepsin digestion and selective salt precipitation. This preparation results in a native conformation of the protein. Antibodies are isolated from rabbit antiserum and are extensively cross-adsorbed by immunoaffinity purification to produce 'type' specific antibodies. Greatly diminished reactivity and selectivity of these antibodies will result if denaturing and reducing conditions of SDS-PAGE and immunoblotting are used.Synonyms: COL4A1

基因ID

9606

UniProt

P02462