Suitable for Immunoblotting, Immunoprecipitation, Immunofluorescence, Flow cytometryand ELISA. Recommended Dilutions: This product was assayed by ELISA against TAP-I peptide in anantibody sandwich assay using Peroxidase conjugated Affinity Purified anti-Rabbit IgG[H&L] (Goat). A dilution of 1: 10,000 is suggested from this experiment. No reaction wasobserved against TAP-II. This product was assayed by immunoblotting and was reactive against the 72 kDa humanTAP-I protein at a dilution of 1: 1,000 followed by reaction with Peroxidase conjugatedAffinity Purified anti-Rabbit IgG [H&L] (Goat). No reaction was observed against TAP-II. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
浓度
1.0 mg/mL
缓冲液
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 containing 0.01 % sodium azide as a preservative.
储存液
Sodium azide
注意事项
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store lyophilized product at 2-8 °C. After reconstitution, store at -20 °C.
抗原
TAP1
(Transporter 1, ATP-Binding Cassette, Sub-Family B (MDR/TAP) (TAP1))