Neutralization. ELISA: To detect hLD78-Beta by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof 0.2-0.4 ng/well of recombinant hLD78-Beta. Western Blot: To detect hLD78-Beta by Western Blot analysis this antibody can be used ata concentration of 0.1- 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hLD78-Beta is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water to a concentration of > 0.2 mg/mL.
缓冲液
PBS, pH 7.2 without preservatives.
储存液
Without preservative
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
LD78 belongs to the intercrine beta (chemokine CC) family. It is closely related to MIP-1 alpha. This protein binds to several chemokine receptors including chemokine binding protein 2 and chemokine (C-C motif) receptor 5 (CCR5). CCR5 is a co-receptor for HIV, binding of this protein to CCR5 inhibits HIV entry, and LD78 beta has been shown to exhibit potent activity in HIV suppression assays. The copy number of the LD78 gene varies among individuals, most individuals have 1-6 copies in the diploid genome, although rare individuals have zero or more than six copies. The gene encodes a 93 amino acid preprotein, the mature protein contains 70 amino acids.Synonyms: C-C motif chemokine 3-like 1, D17S1718, G0/G1 switch regulatory protein 19-2, G0S19-2, SCYA3L1, Small-inducible cytokine A3-like 1, Tonsillar lymphocyte LD78 beta protein