VEGFA 抗体
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- 抗原 See all VEGFA 抗体
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This VEGFA antibody is un-conjugated
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应用范围
- Western Blotting (WB), Enzyme Immunoassay (EIA), Functional Studies (Func)
- 特异性
- This antibody detects Vascular endothelial growth factor A.
- 纯化方法
- Affinity chromatography employing immobilized hVEGF matrix
- 免疫原
- Highly pure (>98%) recombinant hVEGF (human VEGF)
- Top Product
- Discover our top product VEGFA Primary Antibody
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anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 1-147) antibody
VEGFA 适用: 人 WB 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 28-147) antibodyVEGFA 适用: 人 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) antibodyVEGFA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-214) antibodyVEGFA 适用: 大鼠 WB, IHC, IP, ICC 宿主: 小鼠 Monoclonal C2 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA 适用: 小鼠 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-126) antibodyVEGFA 适用: 人 WB, IHC, IF 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-191) antibodyVEGFA 适用: 人, 大鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 33-48), (N-Term) antibodyVEGFA 适用: 人, 大鼠, 小鼠 WB, IHC (p) 宿主: 兔 Polyclonal unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) (AA 27-130) antibodyVEGFA 适用: 人 WB, ELISA, PLA 宿主: 小鼠 Monoclonal 3F7 unconjugated
anti-Vascular Endothelial Growth Factor A (VEGFA) antibodyVEGFA 适用: 人 WB, ELISA, Neut 宿主: 兔 Polyclonal unconjugated
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- 应用备注
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Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhVEGF (10 ng/mL), a concentration of 0.05 - 0.1 μg/mL of this antibody is required. ELISA: Indirect: To detect hVEGF by indirect ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction with compatible secondary reagents, allows the detection of atleast 0.2 - 0.4 ng/well of recombinant hVEGF. Sandwich: To detect hVEGF by sandwich ELISA (using 100 μL/well antibody solution) aconcentration of 0.5 - 2.0 μg/mL of this antibody is required. This antigen affinity purifiedantibody, in conjunction Biotinylated Anti-Human VEGF as a detection antibody, allows thedetection of at least 0.2 - 0.4 ng/well of recombinant hVEGF. Western Blot: To detect hVEGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hVEGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- Lyophilized from PBS, pH 7.2, None
- 注意事项
- This product is photosensitive and should be protected from light. Should it contain a precipitate we recommend microcentrifugation before use.
- 储存条件
- 4 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C. DO NOT FREEZE!
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- 抗原
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- 别名
- VEGF-A (VEGFA 产品)
- 背景
- Synonyms: VEGF, VEGFA, VPF, Vascular endothelial growth factor A, Vascular permeability factor
- 基因ID
- 7422
- NCBI登录号
- NP_001020537
- UniProt
- P15692
- 途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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