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- 抗原 See all FGF7 抗体
- FGF7 (Fibroblast Growth Factor 7 (FGF7))
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适用
- 人
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宿主
- 兔
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克隆类型
- 多克隆
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标记
- This FGF7 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA), Functional Studies (Func)
- 特异性
- This antibody reacts with Human Keratinocyte Growth Factor (KGF).
- 纯化方法
- Affinity chromatography
- 免疫原
- Highly purified (>98%) recombinant FGF-7 (KGF)
- Top Product
- Discover our top product FGF7 Primary Antibody
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- 应用备注
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Neutralisation: To yield one-half maximal inhibition [ND50]of the biological activity of hKGF(25 ng/mL), a concentration of 0.56-0.85 μg/mL of this antibody has been used for previouslots. ELISA: To detect hKGF by direct ELISA (using 100 l/well antibody solution) a concentrationof at least 0.5g/mL of this antibody is required. This antigen affinity purified antibody, inconjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/wellof recombinant hKGF. Western blot: To detect hKGF by Western Blot analysis this antibody can be used at aconcentration of 0.1 - 0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hKGF is 1.5 - 3.0 ng/lane, under either reducing ornon-reducing conditions.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 溶解方式
- Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
- 缓冲液
- PBS, pH 7.2 without preservatives
- 储存液
- Without preservative
- 注意事项
- Avoid repeated freezing and thawing. Centrifuge vial prior to opening!
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.
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Biological and ultrastructural properties of acelagraft, a freeze-dried γ-irradiated human amniotic membrane." in: Archives of ophthalmology, Vol. 128, Issue 10, pp. 1303-10, (2010) (PubMed).
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Biological and ultrastructural properties of acelagraft, a freeze-dried γ-irradiated human amniotic membrane." in: Archives of ophthalmology, Vol. 128, Issue 10, pp. 1303-10, (2010) (PubMed).
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- 抗原
- FGF7 (Fibroblast Growth Factor 7 (FGF7))
- 别名
- FGF7 / KGF (FGF7 产品)
- 别名
- HBGF-7 antibody, KGF antibody, Kgf antibody, kgf antibody, FGF-7 antibody, fgf7 antibody, fibroblast growth factor 7 antibody, fibroblast growth factor 7 L homeolog antibody, FGF7 antibody, Fgf7 antibody, fgf7 antibody, fgf7.L antibody
- 背景
- KGF is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis.Synonyms: Fibroblast growth factor 7, HBGF7, Heparin-binding growth factor 7, Keratinocyte growth factor
- 基因ID
- 2252
- NCBI登录号
- NP_002000
- UniProt
- P21781
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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