Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity ofhGCP-2 (100.0 ng/mL), a concentration of 2.0-5.0 μg/mL of this antibody is required. ELISA: To detect hGCP-2 by direct ELISA (using 100 l/well antibody solution) aconcentration of at least 0.5 μg/mL of this antibody is required. This antigen affinitypurified antibody, in conjunction with compatible secondary reagents, allows the detectionof of 0.2-0.4 ng/well of recombinant hGCP-2. Western Blot: To detect hGCP-2 by Western Blot analysis this antibody can be used at aconcentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary reagentsthe detection limit for recombinant hGCP-2 is 1.5-3.0 ng/lane, under either reducing ornon-reducing conditions. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
缓冲液
PBS, pH 7.2 without preservatives.
储存液
Without preservative
注意事项
Centrifuge vial prior to opening! This product is photosensitive and should be protected from light
Neutrophil recruitment to inflammatory sites is mediated by two related receptors: CXCR1 and CXCR2. Both receptors share two ligands, interleukin-8 (CXCL8) and GCP2 (CXCL6). GCP2 belongs to the intercrine alpha (chemokine CxC) family.Synonyms: C-X-C motif chemokine 6, CKA-3, Chemokine alpha 3, Granulocyte chemotactic protein 2, SCYB6, Small-inducible cytokine B6