ENG
适用: 人
WB, IHC, ELISA
宿主: 兔
Polyclonal
unconjugated
应用备注
ELISA (1 - 15 μg/mL). Western blot (1 - 2 μg/mL). Flow cytometry (3 - 20 μg/mL). Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Reconstitute in sterile water/PBS to a concentration of >0.5 mg/mL
缓冲液
Immunglobulin fraction in Phosphate buffered saline pH 7.4 without preservative and stabilizer
储存液
Without preservative
注意事项
Avoid repeated freeze-thaw cycles. Following reconstitution the antibody is stable for six weeks at 2-8°C.
储存条件
4 °C/-20 °C
储存方法
The lyophilized IgG is stable at 2-8 °C for one month and for one year from despatch when kept at -20 °C.
ENG antibody, MGC137842 antibody, DKFZp469D0419 antibody, END antibody, HHT1 antibody, ORW1 antibody, AI528660 antibody, AI662476 antibody, CD105 antibody, S-endoglin antibody, endoglin antibody, ENG antibody, Eng antibody
背景
Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human endoglin, the S-endoglin and L-endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse endoglin share approximately 70 % and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. It has clearly been shown that CD105/Endoglin is required for angiogenesis and it plays a key role in heart development. Mutations in human endoglin or ALK-1 (another type I serine/threonine receptor) lead to the vascular disorder hereditary hemorrhagic telangiectasia (HHT). Mice heterozygous for endoglin have been developed as disease models for HHT. Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.Synonyms: END, ENG, HHT1, ORW, ORW1