Thrombospondin 1 抗体
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- 抗原 See all Thrombospondin 1 (THBS1) 抗体
- Thrombospondin 1 (THBS1)
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适用
- 哺乳动物
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This Thrombospondin 1 antibody is un-conjugated
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应用范围
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- 特异性
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This antibody is specific to 450 kDa protein (non-reduced) and 170 to 180 kDa (reduced) form of Thrombospondin.
Cellular Localization: Secretory granules, Golgi complex, endoplasmic reticulum - 纯化方法
- Purified
- 免疫原
- BALB/C mice were injected with reduced and alkylated purified human TSP (fully denatured) from the supernatant of thrombin-activated platelets.
- 克隆位点
- A6-1
- 亚型
- IgG1
- Top Product
- Discover our top product THBS1 Primary Antibody
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- 应用备注
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Immunofluorescence. Immunoprecipitation. Western Blot. Immunohistochemistry on paraffin embedded sections: diluted of 1: 25-1: 50 in an ABC method (30 minutes at room temperature). Formalin fixedparaffin embedded tissue sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining. Recommended Positive Control: Tonsil
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Sodium azide as preservative
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- Thrombospondin 1 (THBS1)
- 别名
- Thrombospondin-1 (THBS1) (THBS1 产品)
- 背景
- Thrombospondin is a protein from platelet a granules. It is secreted at sites of platelet activation and aggregation and is involved in the differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.Synonyms: TSP, TSP1
- 基因ID
- 7057
- NCBI登录号
- NP_003237
- UniProt
- P07996
- 途径
- Autophagy
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