Enzyme Immunoassay. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with 1 mL of deionized water and let the lyophilized pellet dissolve completely. Slight turbidity may occur after reconstitution, which does not affect activity of the antibody. In this case clarify the solution by centrifugation.
浓度
1.0 mg/mL
缓冲液
0.05 M phosphate buffer, 0.1 M NaCl, pH 7.2. AZIDE FREE, None
储存液
Azide free
注意事项
Avoid repeated freezing and thawing.
储存条件
-20 °C
储存方法
Prior to reconstitution store at -70 °C. Following reconstitution store the antibody (in aliquots) at -20 °C for 6 month.
TRAP 5 (serum band 5 tartrate-resistant acid phosphatase, TRACP 5, EC 3.1.3.2) is a glycoprotein of 35-37 kDa. TRAP 5 belongs to the most abundant enzymes in osteoclasts. It is expressed in certain differentiated cells of the mononuclear phagocyte system, particularly osteoclasts and alveolar macrophages, where it takes an active part in bone resorption process. High blood levels of TRAP 5 are usually associated with active bone remodelling. Increased serum levels are observed during normal bone growth among healthy children. Elevated serum TRAP levels have been detected in diseases characterized by increased bone resorption, Pagets disease of the bone, hemodialysis, primary hyperparathyroidism, metastatic malignancies involving bone resorption, multiple myeloma and bilaterally ovariectomized women. Post-menopausal women have higher levels of serum than post-menopausal women on estrogen replacement therapy. Therefore specific determination of TRAP 5 activity can be essential for clinical assessment of bone metabolism.Synonyms: Acid phosphatase 5 tartrate resistant, TRAP5, TRAcP, Tartrate-resistant acid ATPase, Tartrate-resistant acid phosphatase type 5