ELISA. Immunoblotting: Recognizes denatured G-CSFR following SDS-PAGE and blotting of 200 μgof cell lysate or following immunoprecipitation with polyclonal antibody followed byimmunoblotting. Western Blot: At an antibody concentration of 1-10 μg/mL will allow visualization of 250 ng/lane of human G-CSFR-MBP. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
限制
仅限研究用
溶解方式
Restore with double distillated water is recommended to adjust the final concentration to 1.00 mg/mL
缓冲液
0.01 M PBS, pH 7.2
注意事项
Avoid repeated freezing and thawing.
储存条件
4 °C/-20 °C
储存方法
Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.
The protein encoded by the GCSF Receptor gene is the receptor for colony stimulating factor 3, a cytokine that controls the production, differentiation, and function of granulocytes. The encoded protein, which is a member of the family of cytokine receptors, may also function in some cell surface adhesion or recognition processes. Four transcript variants encoding four different isoforms have been found for this gene, of which three are membrane-bound and the one secreted and soluble. Mutations in this gene are a cause of Kostmann syndrome, also known as severe congenital neutropenia.Synonyms: G-CSF Receptor, G-CSF-R, GCSF Receptor, GCSFR, Granulocyte colony-stimulating factor receptor