MHC Class I (RT1Ac) 抗体 (FITC)
-
- 抗原 See all MHC Class I (RT1Ac) products
- MHC Class I (RT1Ac)
- 适用
- 大鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- This MHC Class I (RT1Ac) antibody is conjugated to FITC
-
应用范围
- Flow Cytometry (FACS)
- 纯化方法
- Protein G Chromatography.
- 免疫原
- Phytohaemagglutinin Blasts.
- 克隆位点
- OX-27
- 亚型
- IgG2a
-
-
- 应用备注
-
Flow cytometry: use 0.5 g of neat antibody to label 10^6 cells.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Add. Information: Recognizes a polymorphic determinant of the MHC class I antigen in the rat. This Antibody can be used for labelling cells of donor or host origin in bone marrowchimeras. (1,2) - 实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell
- 限制
- 仅限研究用
-
- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS buffer with 0.02 % sodium azide as preservative and 0.5 % EIA grade BSA as stabilizer.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- -20 °C
-
- 抗原
- MHC Class I (RT1Ac)
- Abstract
- MHC Class I (RT1Ac) 产品
- 背景
- MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: MHC class I RT1.Ac heavy chain, RT1-A3
- 基因ID
- 10116
- UniProt
- Q31255
-