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MHC Class I (RT1Ac) 抗体 (FITC)

适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-27 FITC
产品编号 ABIN114394
发货至: 中国
  • 抗原 See all MHC Class I (RT1Ac) products
    MHC Class I (RT1Ac)
    适用
    大鼠
    宿主
    • 11
    小鼠
    克隆类型
    • 11
    单克隆
    标记
    • 4
    • 3
    • 2
    • 2
    This MHC Class I (RT1Ac) antibody is conjugated to FITC
    应用范围
    • 11
    • 4
    • 1
    Flow Cytometry (FACS)
    纯化方法
    Protein G Chromatography.
    免疫原
    Phytohaemagglutinin Blasts.
    克隆位点
    OX-27
    亚型
    IgG2a
  • 应用备注
    Flow cytometry: use 0.5 g of neat antibody to label 10^6 cells.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user. Add. Information: Recognizes a polymorphic determinant of the MHC class I antigen in the rat. This Antibody can be used for labelling cells of donor or host origin in bone marrowchimeras. (1,2)
    实验流程
    FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cell
    限制
    仅限研究用
  • 浓度
    0.1 mg/mL
    缓冲液
    PBS buffer with 0.02 % sodium azide as preservative and 0.5 % EIA grade BSA as stabilizer.
    储存液
    Sodium azide
    注意事项
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    注意事项
    Avoid repeated freezing and thawing.
    储存条件
    -20 °C
  • 抗原
    MHC Class I (RT1Ac)
    Abstract
    MHC Class I (RT1Ac) 产品
    背景
    MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: MHC class I RT1.Ac heavy chain, RT1-A3
    基因ID
    10116
    UniProt
    Q31255
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