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- 抗原 See all MHC Class I (RT1Ac) products
- MHC Class I (RT1Ac)
- 适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This MHC Class I (RT1Ac) antibody is conjugated to Biotin
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应用范围
- Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))
- 纯化方法
- Protein A Chromatography
- 免疫原
- Phytohaemagglutinin Blasts Donor: BALB/c spleen Fusion Partner: P3-NS1-1-Ag4 (NS1/1)
- 克隆位点
- OX-27
- 亚型
- IgG2a
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anti-MHC Class I (RT1Ac) antibody (FITC)
适用: 大鼠 FACS 宿主: 小鼠 Monoclonal OX-27 FITC
anti-MHC Class I (RT1Ac) antibody适用: 大鼠 FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal OX-27 unconjugated
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- 应用备注
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Flow Cytometry. Immunohistochemistry using cryostat sections: (however, cross-reactivity with Lewis ratshave been shown to occur in some instances).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-Rat cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 5-1. 0 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. 7. Wash 2 times at 4°C. 8. Add 100 µl of secondary antibody (Streptavidin-FITC) at a 1: 500 dilution. Notrecommended for use with a PE secondary. 9. Incubate tubes at 4°C for 30 - 60 minutes (It is recommended that tubes are protectedfrom light since most fluorochromes are light sensitive). 10. Wash 2 times at 4°C. 11. Resuspend the cell pellet in 50 µl ice cold media B. 12. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: Brown NorwayCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsIsotypic Control: Biotin Mouse IgG2aCell Source Percentage of cells stained above control: Thymus 65. 4%Spleen 98. 9%Lymph Node 100%Restults - Strain Distribution: Cell Concentration: 1x10e6 cells per testAntibody Concentration Used: 1. 0 µg/10e6 cellsStrains Tested: Brown Norway, Buffalo, Wistar, Fischer, ACIPositive: Brown NorwayNegative: Buffalo, Wistar, Fischer, ACI
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- MHC Class I (RT1Ac)
- Abstract
- MHC Class I (RT1Ac) 产品
- 背景
- MHC Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. MHC class I antigens are heterodimers consisting of one alpha chain (44 kDa) with beta 2 microglobulin (11.5 kDa). The antigen is expressed by all somatic cells at varying levels. MHC Class I molecules are expressed on most nucleated cells where they present endogenously synthesized antigenic peptides to CD8+ T lymphocytes, which are usually cytotoxic T cells. Fibroblasts or neurons however only show a low level of antigen.Synonyms: MHC class I RT1.Ac heavy chain, RT1-A3
- 基因ID
- 10116
- UniProt
- Q31255
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