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- 抗原
- MHC Class II (RT1D)
- 适用
- 大鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- FITC
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应用范围
- Flow Cytometry (FACS)
- 纯化方法
- Protein G Chromatography
- 免疫原
- Rat spleen membrane glycoproteins depleted of Ia-A antigens. Immunocyte Donor: BALB/c spleen Fusion Partner: X63 Ag8.653
- 克隆位点
- OX-17
- 亚型
- IgG1
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anti-MHC Class II (RT1D) antibody (Biotin)
适用: 大鼠 FACS, IHC (fro) 宿主: 小鼠 Monoclonal OX-17 Biotin
anti-MHC Class II (RT1D) antibody适用: 大鼠 FACS, IHC (fro), IP 宿主: 小鼠 Monoclonal OX-17 unconjugated
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- 应用备注
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Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-Rat Cell Separation Medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 05-0. 1 µg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C. (It is recommended that the tubes areprotected from light, since most fluorochromes are light sensitive. )7. Wash 2 times at 4°C. 8. Resuspend the cell pellet in 50 µl ice cold media B. 9. Transfer to suitable tubes for flow cytometric analysis containing 15 µl of propidiumiodide at 0. 5 mg/ml in PBS. This stains dead cells by intercalating in DNA. Media: A. Phosphate buffered saline (pH 7. 2) + 5% normal serum of host species + sodium azide(100 µl of 2M sodium azide in 100 mls). B. Phosphate buffered saline (pH 7. 2) + 0. 5% Bovine serum albumin + sodium azide (100µl of 2M sodium azide in 100 mls). Results - Tissue Distribution: Rat Strain: FischerCell Concentration: 1x10e6 cells per testAntibody Concentration Used: 0. 1 µg/10e6 cellsIsotypic Control: FITC Mouse IgG1Cell Source Percentage of cells stained above control: Thymus 10. 2%Spleen 49. 0%Lymph Node 27. 9%
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- MHC Class II (RT1D)
- 别名
- MHC Class II RT1D
- 背景
- Synonyms: HLA Class II
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