MHC Class II I-Ap 抗体
适用: 小鼠
FACS, CyTox
宿主: 小鼠
Monoclonal
7-16-17
unconjugated
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- 抗原
- MHC Class II I-Ap
- 适用
- 小鼠
-
宿主
- 小鼠
-
克隆类型
- 单克隆
-
标记
- 非结合性
- 应用范围
- Flow Cytometry (FACS), Cytotoxicity Test (CyTox)
- 纯化方法
- Protein G chromatography
- 免疫原
- B10.p
- 克隆位点
- 7-16-17
- 亚型
- IgG2a
-
-
- 应用备注
-
Flow cytometry (protocol see below). Cytotoxic assay.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1x10e6 cells, representing 1 test). 4. To each tube, add 0. 2-1. 0 µg of antibody. 5. Vortex the tubes to ensure thorough mixing of antibody and cells.
- 限制
- 仅限研究用
-
- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS and 0.02 % sodium azide (NaN3).
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
-
- 抗原
- MHC Class II I-Ap
-
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