MHC Class II I-Ap 抗体
适用: 小鼠
FACS, CyTox
宿主: 小鼠
Monoclonal
7-16-17
unconjugated
1 image
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- 抗原
- MHC Class II I-Ap
- 适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Flow Cytometry (FACS), Cytotoxicity Test (CyTox)
- 纯化方法
- Ig Fraction
- 免疫原
- Recipient: BALB/c Donor: B10.P Fusion Partner: Spleen from immunized recipient fused with myeloma SP2/0
- 克隆位点
- 7-16-17
- 亚型
- IgG2a
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- 应用备注
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Cytotoxicity analysis.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF I-A ANTIGEN BEARINGCELLS: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium®1 orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte-M®2 density cell separation medium. After washing, adjustthe cell concentration to 1 x 10e7 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 40 and mix. Alternatively, pellet the cellsand resuspend in antibody diluted 1: 40 in Cytotoxicity Medium. 3. Incubate for 60 minutes at 4°C.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 溶解方式
- Restore with 1.0 mL of distilled water.
- 缓冲液
- PBS
- 注意事项
- Use of sodium azide as a preservative will substantially inhibit the enUse of sodium azide as a preservative will substantially inhibit the enzyme activity of horseradish peroxidase.
- 注意事项
- Avoid repeated freezing and thawing. Should this product contain a precipitate we recommend microcentrifugation before use.
- 储存条件
- -20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- MHC Class II I-Ap
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