MHC Class II I-Abd 抗体
适用: 小鼠
CyTox
宿主: 小鼠
Monoclonal
28-16-8S
unconjugated
1 image
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- 抗原
- MHC Class II I-Abd
- 适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
- 应用范围
- Cytotoxicity Test (CyTox)
- 纯化方法
- Affinity chromatography
- 免疫原
- C3H.SW spleen Donor: C3H Fusion Partner: SP2/0Ag.14
- 克隆位点
- 28-16-8S
- 亚型
- IgM
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anti-MHC Class II I-Abd antibody (Biotin)
适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 28-16-8S Biotin
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- 应用备注
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Flow Cytometry. Can be used to quantitate or eliminate cells bearing the I-Ab antigen from the appropriatestrains of mice.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- CYTOTOXICITY ANALYSIS: Method: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Mediuma orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on Lympholyte®-Mb cell separation medium. After washing, adjust the cellconcentration to 1. 1x10e6 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 640 and mix.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS and contains 0.02 % sodium azide (NaN3) as a preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- MHC Class II I-Abd
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