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- 抗原
- MHC Class II I-Abd
- 适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- Biotin
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应用范围
- Flow Cytometry (FACS)
- 纯化方法
- Affinity chromatography
- 克隆位点
- 28-16-8S
- 亚型
- IgM
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anti-MHC Class II I-Abd antibody
适用: 小鼠 CyTox 宿主: 小鼠 Monoclonal 28-16-8S unconjugated
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- 应用备注
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Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- FLOW CYTOMETRY ANALYSIS: Method: 1. Prepare a cell suspension in media A. For cell preparations, deplete the red blood cellpopulation with Lympholyte®-M cell separation medium. 2. Wash 2 times. 3. Resuspend the cells to a concentration of 2x10e7 cells/ml in media A. Add 50 µl of thissuspension to each tube (each tube will then contain 1 x 10e6 cells, representing 1 test). 4. To each tube, add 0. 2µg - 0. 5mg* of this Ab per 10e6 cells. 5. Vortex the tubes to ensure thorough mixing of antibody and cells. 6. Incubate the tubes for 30 minutes at 4°C.
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS and contains 0.02 % sodium azide (NaN3) as a preservative.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- MHC Class II I-Abd
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