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- 抗原
- MHC Class I (H-2Kb/H-2Db)
- 适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- 非结合性
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应用范围
- Cytotoxicity Test (CyTox)
- 纯化方法
- Protein G chromatography
- 克隆位点
- 5041-16-1
- 亚型
- IgG2a
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- 应用备注
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Cytotoxicity assay (see protocol).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- RECOMMENDED METHOD FOR DEPLETING A CELL POPULATION OF H-2Kb AND/OR H-2Db BEARING LYMPHOCYTES: 1. Prepare a cell suspension from the appropriate tissue in Cytotoxicity Medium1 orequivalent. Remove red cells and dead cells (where necessary) by purification of viablelymphocytes on density cell separation medium. After washing, adjust the cellconcentration to 1x10e7 cells per ml in Cytotoxicity Medium. 2. Add the antibody to a final concentration of 1: 100 and mix. Alternatively, pellet the cellsand resuspend in antibody diluted 1: 100 in Cytotoxicity Medium. 3. Incubate for 60 minutes at 4°C. 4. Centrifuge to pellet the cells and discard the supernatant. 5. Resuspend to the original volume in Rabbit Complement, diluted to the appropriateconcentration in Cytotoxicity Medium. (Recommended concentration included with eachbatch of Rabbit Complement. )6. Incubate for 60 minutes at 37°C. 7. Monitor for percent cytotoxicity at this stage, before further processing. For this purposeremove a small sample from each tube, dilute 1: 10 with medium, and add 1/10 volumeof 1% Trypan Blue. After 3-5 minutes, score live versus dead cells in a haemacytometer.
- 限制
- 仅限研究用
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- 浓度
- 1.0 mg/mL
- 缓冲液
- PBS + 0.02 % NaN3
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. Dilute only prior to immediate use
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody (undiluted) at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- MHC Class I (H-2Kb/H-2Db)
- 别名
- MHC Class I H-2 Kb / H-2 Db
- 背景
- Synonyms: HLA Class I
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