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- 抗原 See all H-2Dd products
- H-2Dd (MHC Class I H-2Dd)
- 适用
- 小鼠
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宿主
- 小鼠
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克隆类型
- 单克隆
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标记
- This H-2Dd antibody is conjugated to FITC
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应用范围
- Flow Cytometry (FACS)
- 特异性
- This anti-mouse H-2Dd monoclonal antibody is specific for cells expressing the H-2D antigen coded for by the d haplotype. The reaction pattern of this antibody with a panel of inbred and recombinant haplotypes demonstrates that the antibody detects a private determinant (H-2.4) of the H-2Dd antigen. This antibody can be used to quantitate cells bearing the H-2Dd(H-2.4) antigen from the appropriate strains of mice.
- 纯化方法
- Protein G Chromatography
- 免疫原
- Recipient: C3H/HeJ Immunocyte Donor: B6 x DBA/2 spleen cells Fusion Partner: SP2/0.Ag14
- 克隆位点
- 34-5-8S
- 亚型
- IgG2a
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anti-MHC Class I H-2Dd antibody
适用: 小鼠 FACS, IP 宿主: 小鼠 Monoclonal 34-5-8S unconjugated
anti-MHC Class I H-2Dd antibody (Biotin)适用: 小鼠 FACS, IP 宿主: 小鼠 Monoclonal 34-5-8S Biotin
anti-MHC Class I H-2Dd antibody (PE)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 34-5-8S PE
anti-MHC Class I H-2Dd antibody (FITC)适用: 小鼠 FACS 宿主: 小鼠 Monoclonal 34-5-8S FITC
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- 应用备注
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Flow cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - 实验流程
- FLOW CYTOMETRY ANALYSIS:
- 限制
- 仅限研究用
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- 浓度
- 0.1 mg/mL
- 缓冲液
- PBS, 0.02 % NaN3 and EIA grade BSA as a stabilizing protein to bring total protein concentration to 4-5 mg/mL.
- 储存液
- Sodium azide
- 注意事项
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 注意事项
- Avoid repeated freezing and thawing. This product is photosensitive and should be protected from light.
- 储存条件
- 4 °C/-20 °C
- 储存方法
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- 抗原
- H-2Dd (MHC Class I H-2Dd)
- 别名
- MHC Class I H-2 Dd (H-2Dd 产品)
- 背景
- Synonyms: D-D alpha chain, H-2 class I histocompatibility antigen, H-2D(D), H2-D1
- 基因ID
- 100045864
- NCBI登录号
- XP_003086970
- UniProt
- P01900
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